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Genes 2018, 9(5), 256; https://doi.org/10.3390/genes9050256

Characterization of Antimicrobial Resistance Determinants and Class 1 and Class 2 Integrons in Salmonella enterica spp., Multidrug-Resistant Isolates from Pigs

1
Group of Genomics and Animal breeding, Department of Genetics, Faculty of Veterinary Medicine, University of Córdoba, 14071 Córdoba, Spain
2
Department of Animal Health, Faculty of Veterinary Medicine, University of León, 24007 León, Spain
3
German Federal Institute for Risk Assessment, 10589 Berlin, Germany
4
European Food Safety Authority, 43126 Parma, Italy
*
Author to whom correspondence should be addressed.
Received: 27 March 2018 / Revised: 2 May 2018 / Accepted: 8 May 2018 / Published: 16 May 2018
(This article belongs to the Special Issue Genetics and Genomics of Foodborne Pathogens)
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Abstract

Antimicrobial resistance (AMR) and Salmonella spp., are primary concerns in public health. The present study characterizes the AMR determinants of 62 multi-drug resistant (MDR) Salmonella enterica spp., isolates from swine, which were obtained between 2004–2006, a major source of human salmonellosis. The AMR determinants were investigated by PCR, checking the presence of class 1 and class 2 integrons and 29 resistance genes. Genes sul1, blaTEM1-like, aadA2, tet(A), and dfrA12 were more prevalent (p < 0.05) within the determinants that were checked for each of these antimicrobials. Co-existence of different genes conferring resistance to the same antimicrobial was common. No differences in AMR determinants prevalence were observed between Salmonella Typhimurium and other serovars from the study. Class 1 integrons were detected in 48 of 62 isolates, again with no differences being linked to any serovar. Nine different variable regions were observed, 1000 bp/aadA2-1200 bp/blaPSE-1 (13 isolates) and blaOXA-like/aadA1 (eight isolates) were the most common. Four isolates, including S. Typhimurium (2), Salmonella Bredeney (1), and Salmonella Kapemba (1) harboured a class 2 integron 2300 bp estX-sat2-aadA1. Results from the study highlight the importance of class 1 integrons and certain genes in MDR swine Salmonella isolates. The information is of relevance for monitoring in the forthcoming scope of reduction of antibiotic usage in swine production. View Full-Text
Keywords: resistance gene; swine; food-borne pathogen; public health; antibiotic resistance gene; swine; food-borne pathogen; public health; antibiotic
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Argüello, H.; Guerra, B.; Rodríguez, I.; Rubio, P.; Carvajal, A. Characterization of Antimicrobial Resistance Determinants and Class 1 and Class 2 Integrons in Salmonella enterica spp., Multidrug-Resistant Isolates from Pigs. Genes 2018, 9, 256.

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