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Article

Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro

1
ICAR-Central Inland Fisheries Research Institute, Biochemistry Laboratory, Proteomics Unit, Barrackpore, Kolkata 700120, India
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Department of Pharmacology and Toxicology, College of Veterinary Sciences and Animal Husbandry, Orissa University of Agriculture and Technology, Bhubaneswar 751003, India
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Departments of Pediatrics, Children’s Hospital of Philadelphia Research Institute, The Raymond and Ruth Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA
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ICAR-National Rice Research Institute, Cuttack 753006, India
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Department of Biotechnology, Faculty of Science & Technology, Rama Devi Women’s University, Bhubaneswar 751022, India
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Department of Obstetrics and Gynecology, UMKC School of Medicine, Kansas City, MO 64108, USA
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Indian Council of Agricultural Research (ICAR), ICAR-Fisheries Science Division, Room No. 308, Krishi Anusandhan Bhawan II, New Delhi 110012, India
*
Authors to whom correspondence should be addressed.
Academic Editor: Gary Sutkin
Genes 2022, 13(5), 801; https://doi.org/10.3390/genes13050801
Received: 31 March 2022 / Revised: 23 April 2022 / Accepted: 24 April 2022 / Published: 29 April 2022
Although metabolic acidosis is associated with numerous pathophysiological conditions and its vasorelaxation effects have been well described in different animal and culture models, the molecular mechanisms of acidosis-induced vasorelaxation are not fully understood. Mesenteric artery models have been used extensively to examine the vascular response to various pathophysiological conditions. Our previous studies and several other reports have suggested the vascular responses of goat mesenteric arteries and human arteries to various stimuli, including acidic stress, are highly similar. In this study, to further identify the signaling molecules responsible for altered vasoreactivity in response to acidic pH, we examined the proteomic profile of acid stress-induced vasorelaxation using a goat mesenteric artery model. The vascular proteomes under acidic pH were compared using 2D-GE with 7 cm IPG strips and mini gels, LC-MS/MS, and MALDI TOF MS. The unique proteins identified by mass spectroscopy were actin, transgelin, WD repeat-containing protein 1, desmin, tropomyosin, ATP synthase β, Hsp27, aldehyde dehydrogenase, pyruvate kinase, and vitamin K epoxide reductase complex subunit 1-like protein. Out of five protein spots identified as actin, three were upregulated > 2-fold. ATP synthase β was also upregulated (2.14-fold) under acid stress. Other actin-associated proteins upregulated were transgelin, desmin, and WD repeat-containing protein 1. Isometric contraction studies revealed that both receptor-mediated (histamine) and non-receptor-mediated (KCl) vasocontraction were attenuated, whereas acetylcholine-induced vasorelaxation was augmented under acidosis. Overall, the altered vasoreactivity under acidosis observed in the functional studies could possibly be attributed to the increase in expression of actin and ATP synthase β. View Full-Text
Keywords: acid stress; actin regulation; ATP synthetase β; mesenteric artery; metabolic acidosis; proteome; vascular tone; vasorelaxation acid stress; actin regulation; ATP synthetase β; mesenteric artery; metabolic acidosis; proteome; vascular tone; vasorelaxation
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MDPI and ACS Style

Mohanty, I.; Banerjee, S.; Mahanty, A.; Mohanty, S.; Nayak, N.R.; Parija, S.C.; Mohanty, B.P. Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro. Genes 2022, 13, 801. https://doi.org/10.3390/genes13050801

AMA Style

Mohanty I, Banerjee S, Mahanty A, Mohanty S, Nayak NR, Parija SC, Mohanty BP. Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro. Genes. 2022; 13(5):801. https://doi.org/10.3390/genes13050801

Chicago/Turabian Style

Mohanty, Ipsita, Sudeshna Banerjee, Arabinda Mahanty, Sasmita Mohanty, Nihar Ranjan Nayak, Subas Chandra Parija, and Bimal Prasanna Mohanty. 2022. "Proteomic Profiling and Pathway Analysis of Acid Stress-Induced Vasorelaxation of Mesenteric Arteries In Vitro" Genes 13, no. 5: 801. https://doi.org/10.3390/genes13050801

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