Next Article in Journal
Genome-Wide Identification, Expression Profile of the TIFY Gene Family in Brassica oleracea var. capitata, and Their Divergent Response to Various Pathogen Infections and Phytohormone Treatments
Previous Article in Journal
Imaging Mitochondrial Functions: From Fluorescent Dyes to Genetically-Encoded Sensors
Open AccessArticle

Pulmonary Hypertension Remodels the Genomic Fabrics of Major Functional Pathways

1
Department of Pediatrics, New York Medical College, Valhalla, NY 10595, USA
2
Department of Physiology, New York Medical College, Valhalla, NY 10595, USA
3
Department of Pathology, New York Medical College, Valhalla, NY 10595, USA
4
Personalized Genomics Laboratory, Center for Computational Systems Biology, Roy G Perry College of Engineering, Prairie View A&M University, Prairie View, TX 77446, USA
*
Author to whom correspondence should be addressed.
Genes 2020, 11(2), 126; https://doi.org/10.3390/genes11020126 (registering DOI)
Received: 17 December 2019 / Revised: 17 January 2020 / Accepted: 21 January 2020 / Published: 23 January 2020
(This article belongs to the Section Human Genomics and Genetic Diseases)
Pulmonary hypertension (PH) is a serious disorder with high morbidity and mortality rate. We analyzed the right-ventricular systolic pressure (RVSP), right-ventricular hypertrophy (RVH), lung histology, and transcriptomes of six-week-old male rats with PH induced by (1) hypoxia (HO), (2) administration of monocrotaline (CM), or (3) administration of monocrotaline and exposure to hypoxia (HM). The results in PH rats were compared to those in control rats (CO). After four weeks exposure, increased RVSP and RVH, pulmonary arterial wall thickening, and alteration of the lung transcriptome were observed in all PH groups. The HM group exhibited the largest alterations, as well as neointimal lesions and obliteration of the lumen in small arteries. We found that PH increased the expression of caveolin1, matrix metallopeptidase 2, and numerous inflammatory and cell proliferation genes. The cell cycle, vascular smooth muscle contraction, and oxidative phosphorylation pathways, as well as their interplay, were largely perturbed. Our results also suggest that the upregulated Rhoa (Ras homolog family member A) mediates its action through expression coordination with several ATPases. The upregulation of antioxidant genes and the extensive mitochondrial damage observed, especially in the HM group, indicate metabolic shift toward aerobic glycolysis. View Full-Text
Keywords: aerobic glycolysis; caveolin1; hypoxia; monocrotaline; oxidative phosphorylation; RhoA aerobic glycolysis; caveolin1; hypoxia; monocrotaline; oxidative phosphorylation; RhoA
Show Figures

Figure 1

MDPI and ACS Style

Mathew, R.; Huang, J.; Iacobas, S.; Iacobas, D.A. Pulmonary Hypertension Remodels the Genomic Fabrics of Major Functional Pathways. Genes 2020, 11, 126.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop