Next Article in Journal
The Emerging Role of Long Non-Coding RNAs in Development and Function of Gilthead Sea Bream (Sparus aurata) Fast Skeletal Muscle
Previous Article in Journal
Therapeutic Targeting of Intestinal Fibrosis in Crohn’s Disease
Previous Article in Special Issue
Scaffold-Mediated Immunoengineering as Innovative Strategy for Tendon Regeneration
Article

Influence of ROCK Pathway Manipulation on the Actin Cytoskeleton Height

Institute of Experimental Physics, University Ulm, 89081 Ulm, Baden-Württemberg, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Alberto Sensini, Gordon Blunn, Martijn van Griensven and Lorenzo Moroni
Cells 2022, 11(3), 430; https://doi.org/10.3390/cells11030430
Received: 13 December 2021 / Revised: 22 January 2022 / Accepted: 24 January 2022 / Published: 26 January 2022
The actin cytoskeleton with its dynamic properties serves as the driving force for the movement and division of cells and gives the cell shape and structure. Disorders in the actin cytoskeleton occur in many diseases. Deeper understanding of its regulation is essential in order to better understand these biochemical processes. In our study, we use metal-induced energy transfer (MIET) as a tool to quantitatively examine the rarely considered third dimension of the actin cytoskeleton with nanometer accuracy. In particular, we investigate the influence of different drugs acting on the ROCK pathway on the three-dimensional actin organization. We find that cells treated with inhibitors have a lower actin height to the substrate while treatment with a stimulator for the ROCK pathway increases the actin height to the substrate, while the height of the membrane remains unchanged. This reveals the precise tuning of adhesion and cytoskeleton tension, which leads to a rich three-dimensional structural behaviour of the actin cytoskeleton. This finetuning is differentially affected by either inhibition or stimulation. The high axial resolution shows the importance of the precise finetuning of the actin cytoskeleton and the disturbed regulation of the ROCK pathway has a significant impact on the actin behavior in the z dimension. View Full-Text
Keywords: actin cytoskeleton; ROCK pathway; metal-induced energy transfer; z-dimension actin cytoskeleton; ROCK pathway; metal-induced energy transfer; z-dimension
Show Figures

Figure 1

MDPI and ACS Style

Grandy, C.; Port, F.; Pfeil, J.; Gottschalk, K.-E. Influence of ROCK Pathway Manipulation on the Actin Cytoskeleton Height. Cells 2022, 11, 430. https://doi.org/10.3390/cells11030430

AMA Style

Grandy C, Port F, Pfeil J, Gottschalk K-E. Influence of ROCK Pathway Manipulation on the Actin Cytoskeleton Height. Cells. 2022; 11(3):430. https://doi.org/10.3390/cells11030430

Chicago/Turabian Style

Grandy, Carolin, Fabian Port, Jonas Pfeil, and Kay-Eberhard Gottschalk. 2022. "Influence of ROCK Pathway Manipulation on the Actin Cytoskeleton Height" Cells 11, no. 3: 430. https://doi.org/10.3390/cells11030430

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop