Proteomic Analysis of Niemann-Pick Type C Hepatocytes Reveals Potential Therapeutic Targets for Liver Damage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Primary Mouse Hepatocytes Isolation
2.2. Proteomic Sample Preparation
2.3. LC-MS/MS Analysis
2.4. Data Analysis
2.5. Upstream Regulator Analysis
2.6. Western Blot Analysis
3. Results
3.1. NPC1 Deficient Hepatocytes Show 416 Proteins with Significant Expression Changes
3.2. Differentially Expressed Proteins in NPC1 Deficient Hepatocytes Are Predominantly Related to Alterations in Metabolism and Liver Function
3.3. Lysosomal and Mitochondrial Proteins Are Differentially Expressed in NPC1 Deficient Hepatocytes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Balboa, E.; Marín, T.; Oyarzún, J.E.; Contreras, P.S.; Hardt, R.; van den Bosch, T.; Alvarez, A.R.; Rebolledo-Jaramillo, B.; Klein, A.D.; Winter, D.; et al. Proteomic Analysis of Niemann-Pick Type C Hepatocytes Reveals Potential Therapeutic Targets for Liver Damage. Cells 2021, 10, 2159. https://doi.org/10.3390/cells10082159
Balboa E, Marín T, Oyarzún JE, Contreras PS, Hardt R, van den Bosch T, Alvarez AR, Rebolledo-Jaramillo B, Klein AD, Winter D, et al. Proteomic Analysis of Niemann-Pick Type C Hepatocytes Reveals Potential Therapeutic Targets for Liver Damage. Cells. 2021; 10(8):2159. https://doi.org/10.3390/cells10082159
Chicago/Turabian StyleBalboa, Elisa, Tamara Marín, Juan Esteban Oyarzún, Pablo S. Contreras, Robert Hardt, Thea van den Bosch, Alejandra R. Alvarez, Boris Rebolledo-Jaramillo, Andres D. Klein, Dominic Winter, and et al. 2021. "Proteomic Analysis of Niemann-Pick Type C Hepatocytes Reveals Potential Therapeutic Targets for Liver Damage" Cells 10, no. 8: 2159. https://doi.org/10.3390/cells10082159