Next Article in Journal
Phosphorylation in the Charged Linker Modulates Interactions and Secretion of Hsp90β
Next Article in Special Issue
The Role of Immunotherapy in a Tolerogenic Environment: Current and Future Perspectives for Hepatocellular Carcinoma
Previous Article in Journal
A Bimodal Fluorescence-Raman Probe for Cellular Imaging
Article

Eg5 as a Prognostic Biomarker and Potential Therapeutic Target for Hepatocellular Carcinoma

1
Graduate Institute of Oncology, National Taiwan University College of Medicine, 1, Sec. 1, Ren’ai Rd., Taipei 100, Taiwan
2
Department of Oncology, National Taiwan University Hospital, 7, Chung-Shan S. Rd., Taipei 100, Taiwan
3
Department of Pathology, National Taiwan University Hospital, 7, Chung-Shan S. Rd., Taipei 100, Taiwan
4
Graduate Institute of Pathology, National Taiwan University College of Medicine, 1, Sec. 1, Ren’ai Rd., Taipei 100, Taiwan
5
Department of Surgery, National Taiwan University Hospital, 7, Chung-Shan S. Rd., Taipei 100, Taiwan
6
Department of Medical Oncology, National Taiwan University Cancer Center, 57, Lane 155, Sec. 3, Keelung Rd., Taipei 106, Taiwan
7
Department of Internal Medicine, National Taiwan University Hospital, 7, Chung-Shan S. Rd., Taipei 100, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editors: Kyu Yun Jang and See-Hyoung Park
Cells 2021, 10(7), 1698; https://doi.org/10.3390/cells10071698
Received: 24 May 2021 / Revised: 29 June 2021 / Accepted: 1 July 2021 / Published: 5 July 2021
(This article belongs to the Special Issue Molecular Mechanisms of Hepatocellular Carcinoma)
Background: The kinesin Eg5, a mitosis-associated protein, is overexpressed in many cancers. Here we explored the clinical significance of Eg5 in hepatocellular carcinoma (HCC). Methods: HCC tissues from surgical resection were collected. Total RNA was prepared from tumorous and nontumorous parts. Eg5 expression levels were correlated with overall survival (OS) and disease-free survival (DFS). In vitro efficacy of LGI-147, a specific Eg5 inhibitor, was tested in HCC cell lines. In vivo efficacy of Eg5 inhibition was investigated in a xenograft model. Results: A total of 108 HCC samples were included. The patients were divided into three tertile groups with high, medium, and low Eg5 expression levels. OS of patients with low Eg5 expression was better than that of patients with medium and high Eg5 expression (median, 155.6 vs. 75.3 vs. 57.7 months, p = 0.002). DFS of patients with low Eg5 expression was also better than that of patients with medium and high Eg5 expression (median, 126.3 vs. 46.2 vs. 39.4 months, p = 0.001). In multivariate analyses, the associations between Eg5 expression and OS (p < 0.001) or DFS remained (p < 0.001). LGI-147 reduced cell growth via cell cycle arrest and apoptosis and induced accumulation of abnormal mitotic cells. In the xenograft model, the tumor growth rate under LGI-147 treatment was significantly slower than under the control. Conclusion: High Eg5 expression was associated with poor HCC prognosis. In vitro and in vivo evidence suggests that Eg5 may be a reasonable therapeutic target for HCC. View Full-Text
Keywords: Eg5; hepatocellular carcinoma; kinesin; mitosis; prognosis Eg5; hepatocellular carcinoma; kinesin; mitosis; prognosis
Show Figures

Figure 1

MDPI and ACS Style

Shao, Y.-Y.; Sun, N.-Y.; Jeng, Y.-M.; Wu, Y.-M.; Hsu, C.; Hsu, C.-H.; Hsu, H.-C.; Cheng, A.-L.; Lin, Z.-Z. Eg5 as a Prognostic Biomarker and Potential Therapeutic Target for Hepatocellular Carcinoma. Cells 2021, 10, 1698. https://doi.org/10.3390/cells10071698

AMA Style

Shao Y-Y, Sun N-Y, Jeng Y-M, Wu Y-M, Hsu C, Hsu C-H, Hsu H-C, Cheng A-L, Lin Z-Z. Eg5 as a Prognostic Biomarker and Potential Therapeutic Target for Hepatocellular Carcinoma. Cells. 2021; 10(7):1698. https://doi.org/10.3390/cells10071698

Chicago/Turabian Style

Shao, Yu-Yun, Nai-Yun Sun, Yung-Ming Jeng, Yao-Ming Wu, Chiun Hsu, Chih-Hung Hsu, Hey-Chi Hsu, Ann-Lii Cheng, and Zhong-Zhe Lin. 2021. "Eg5 as a Prognostic Biomarker and Potential Therapeutic Target for Hepatocellular Carcinoma" Cells 10, no. 7: 1698. https://doi.org/10.3390/cells10071698

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop