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Open AccessArticle

Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9)

1
Institute of Anatomy II, Goethe-University, D-60590 Frankfurt am Main, Germany
2
Department of Neurosurgery, Rostock University Medical Center, D-18055 Rostock, Germany
*
Author to whom correspondence should be addressed.
Cells 2021, 10(1), 72; https://doi.org/10.3390/cells10010072
Received: 12 August 2020 / Revised: 28 December 2020 / Accepted: 30 December 2020 / Published: 5 January 2021
(This article belongs to the Special Issue Neuron-Glia Interactions)
Astrocytes contribute to many higher brain functions. A key mechanism in glia-to-neuron signalling is vesicular exocytosis; however, the identity of exocytosis organelles remains a matter of debate. Since vesicles derived from the trans-Golgi network (TGN) are not considered in this context, we studied the astrocyte TGN by immunocytochemistry applying anti-Rab6A. In mouse brain, Rab6A immunostaining is found to be unexpectedly massive, diffuse in all regions, and is detected preferentially and abundantly in the peripheral astrocyte processes, which is hardly evident without glial fibrillary acid protein (GFAP) co-staining. All cells positive for the astrocytic markers glutamine synthetase (GS), GFAP, aldehyde dehydrogenase 1 family member L1 (Aldh1L1), or SRY (sex determining region Y)-box 9 (SOX9) were Rab6A+. Rab6A is excluded from microglia, oligodendrocytes, and NG2 cells using cell type-specific markers. In human cortex, Rab6A labelling is very similar and associated with GFAP+ astrocytes. The mouse data also confirm the specific astrocytic labelling by Aldh1L1 or SOX9; the astrocyte-specific labelling by GS sometimes debated is replicated again. In mouse and human brain, individual astrocytes display high variability in Rab6A+ structures, suggesting dynamic regulation of the glial TGN. In summary, Rab6A expression is an additional, global descriptor of astrocyte identity. Rab6A might constitute an organelle system with a potential role of Rab6A in neuropathological and physiological processes. View Full-Text
Keywords: trans-Golgi network; astrocyte heterogeneity; immunocytochemistry trans-Golgi network; astrocyte heterogeneity; immunocytochemistry
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MDPI and ACS Style

Melzer, L.; Freiman, T.M.; Derouiche, A. Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9). Cells 2021, 10, 72. https://doi.org/10.3390/cells10010072

AMA Style

Melzer L, Freiman TM, Derouiche A. Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9). Cells. 2021; 10(1):72. https://doi.org/10.3390/cells10010072

Chicago/Turabian Style

Melzer, Linda; Freiman, Thomas M.; Derouiche, Amin. 2021. "Rab6A as a Pan-Astrocytic Marker in Mouse and Human Brain, and Comparison with Other Glial Markers (GFAP, GS, Aldh1L1, SOX9)" Cells 10, no. 1: 72. https://doi.org/10.3390/cells10010072

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