Efficient Protocol for the In Vitro Plantlet Production of Caper (Capparis orientalis Veill.) from the East Adriatic Coast

Kereša, Snježana; Stanković, Davor; Batelja Lodeta, Kristina; Habuš Jerčić, Ivanka; Bolarić, Snježana; Barić, Marijana; Bošnjak Mihovilović, Anita

doi:10.3390/agronomy9060303

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Efficient Protocol for the In Vitro Plantlet Production of Caper (Capparis orientalis Veill.) from the East Adriatic Coast

Snježana Kereša

^1,*

Davor Stanković

²,

Kristina Batelja Lodeta

Ivanka Habuš Jerčić

¹,

Snježana Bolarić

¹,

Marijana Barić

¹ and

Anita Bošnjak Mihovilović

Department of Plant Breeding, Genetics and Biometrics, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, Zagreb 10000, Croatia

Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, Zagreb 10000, Croatia

Department of Pomology, Faculty of Agriculture, University of Zagreb, Svetošimunska cesta 25, Zagreb 10000, Croatia

Author to whom correspondence should be addressed.

Agronomy 2019, 9(6), 303; https://doi.org/10.3390/agronomy9060303

Received: 8 May 2019 / Revised: 3 June 2019 / Accepted: 9 June 2019 / Published: 11 June 2019

(This article belongs to the Section Horticultural and Floricultural Crops)

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Abstract

Caper (Capparis orientalis Veill.) is a species rich in bioactive compounds, with positive effects on human health. It has a great adaptability to harsh environments and an exceptional ability to extract water from dry soils. In Croatia, the caper grows as a wild plant, and its cultivation is insignificant, which is probably due to propagation difficulties. Micropropagation could be a solution for this. The aim of this study was to investigate the success of the micropropagation, in vitro rooting, and acclimatization of Capparis orientalis Veill. Shoot proliferation was tested in a Murashige and Skoog (MS) medium, with sucrose or glucose, and in 13 treatments, presenting the combined effect of different cytokinins and their concentrations. The success of rooting was examined in relation to the impact of various auxins, durations of rooting, and carry-over effects. A better proliferation was achieved when sucrose was used. The highest number (18) of shoots/explants was obtained in the medium supplemented with 0.6 mg·L⁻¹ meta-topolin, while the rooting was equally efficient in the media supplemented with 2 mg·L⁻¹ of indole-3-acetic acid or indole-3-butyric acid, or in hormone-free rooting medium. A prolonged time in the media increased the rooting efficiency, while the carry-over effect had no influence. The acclimatization rate reached 66%. Additional efforts should be made to find out how to speed upthe rooting and enhance the acclimatization rate of caper grown in Croatia. View Full-Text

Keywords: caper; micropropagation; rooting; cytokinins; auxins

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Kereša, S.; Stanković, D.; Batelja Lodeta, K.; Habuš Jerčić, I.; Bolarić, S.; Barić, M.; Bošnjak Mihovilović, A. Efficient Protocol for the In Vitro Plantlet Production of Caper (Capparis orientalis Veill.) from the East Adriatic Coast. Agronomy 2019, 9, 303. https://doi.org/10.3390/agronomy9060303

AMA Style

Kereša S, Stanković D, Batelja Lodeta K, Habuš Jerčić I, Bolarić S, Barić M, Bošnjak Mihovilović A. Efficient Protocol for the In Vitro Plantlet Production of Caper (Capparis orientalis Veill.) from the East Adriatic Coast. Agronomy. 2019; 9(6):303. https://doi.org/10.3390/agronomy9060303

Chicago/Turabian Style

Kereša, Snježana, Davor Stanković, Kristina Batelja Lodeta, Ivanka Habuš Jerčić, Snježana Bolarić, Marijana Barić, and Anita Bošnjak Mihovilović. 2019. "Efficient Protocol for the In Vitro Plantlet Production of Caper (Capparis orientalis Veill.) from the East Adriatic Coast" Agronomy 9, no. 6: 303. https://doi.org/10.3390/agronomy9060303

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Efficient Protocol for the In Vitro Plantlet Production of Caper (Capparis orientalis Veill.) from the East Adriatic Coast

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