Next Article in Journal
Performance Evaluation of Cellulose Nanofiber with Residual Hemicellulose as a Nanofiller in Polypropylene-Based Nanocomposite
Next Article in Special Issue
Polyelectrolyte Multilayers on Soft Colloidal Nanosurfaces: A New Life for the Layer-By-Layer Method
Previous Article in Journal
Controlled SrR Delivery by the Incorporation of Mg Particles on Biodegradable PLA-Based Composites
Previous Article in Special Issue
Gas Separation by Mixed Matrix Membranes with Porous Organic Polymer Inclusions within o-Hydroxypolyamides Containing m-Terphenyl Moieties
Article

Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts

1
Colegio Máximo de Cartuja s/n, Faculty of Dentistry, University of Granada, 18071 Granada, Spain
2
Medicina Clínica y Salud Pública Programme, University of Granada, 18071 Granada, Spain
3
Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, 18071 Granada, Spain
4
Instituto Investigación Biosanitaria, ibs.Granada, 18071 Granada, Spain
5
NanoMyP®, Nanomateriales y Polimeros S.L., Spin-Off Company of the University of Granada, Edificio BIC-Granada, Av. Innovación 1, Armilla, 18016 Granada, Spain
6
Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, 18071 Granada, Spain
7
Institute of Neuroscience, University of Granada, Centro de Investigación Biomédica (CIBM), Parque de Tecnológico de la Salud (PTS), 18071 Granada, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Rebeca Hernandez Velasco
Polymers 2021, 13(7), 1063; https://doi.org/10.3390/polym13071063
Received: 9 March 2021 / Revised: 23 March 2021 / Accepted: 25 March 2021 / Published: 28 March 2021
(This article belongs to the Special Issue State-of-the-Art Polymer Science and Technology in Spain (2020,2021))
Polymeric membranes are employed in guided bone regeneration (GBR) as physical barriers to facilitate bone in-growth. A bioactive and biomimetic membrane with the ability to participate in the healing and regeneration of the bone is necessary. The aim of the present study was to analyze how novel silicon dioxide composite membranes functionalized with zinc or doxycycline can modulate the osteoblasts’ proliferation, differentiation, and expression of selected antigenic markers related to immunomodulation. Nanostructured acrylate-based membranes were developed, blended with silica, and functionalized with zinc or doxycycline. They were subjected to MG63 osteoblast-like cells culturing. Proliferation was assessed by MTT-assay, differentiation by evaluating the alkaline phosphatase activity by a spectrophotometric method and antigenic phenotype was assessed by flow cytometry for selected markers. Mean comparisons were conducted by one-way ANOVA and Tukey tests (p < 0.05). The blending of silica nanoparticles in the tested non-resorbable polymeric scaffold improved the proliferation and differentiation of osteoblasts, but doxycycline doped scaffolds attained the best results. Osteoblasts cultured on doxycycline functionalized membranes presented higher expression of CD54, CD80, CD86, and HLA-DR, indicating a beneficial immunomodulation activity. Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal disease. View Full-Text
Keywords: CD markers; doxycycline; antigenic phenotype; osteoblasts; membrane CD markers; doxycycline; antigenic phenotype; osteoblasts; membrane
Show Figures

Figure 1

MDPI and ACS Style

Toledano-Osorio, M.; Manzano-Moreno, F.J.; Toledano, M.; Medina-Castillo, A.L.; Costela-Ruiz, V.J.; Ruiz, C.; Osorio, R. Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts. Polymers 2021, 13, 1063. https://doi.org/10.3390/polym13071063

AMA Style

Toledano-Osorio M, Manzano-Moreno FJ, Toledano M, Medina-Castillo AL, Costela-Ruiz VJ, Ruiz C, Osorio R. Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts. Polymers. 2021; 13(7):1063. https://doi.org/10.3390/polym13071063

Chicago/Turabian Style

Toledano-Osorio, Manuel, Francisco J. Manzano-Moreno, Manuel Toledano, Antonio L. Medina-Castillo, Victor J. Costela-Ruiz, Concepción Ruiz, and Raquel Osorio. 2021. "Doxycycline-Doped Polymeric Membranes Induced Growth, Differentiation and Expression of Antigenic Phenotype Markers of Osteoblasts" Polymers 13, no. 7: 1063. https://doi.org/10.3390/polym13071063

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop