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Article

Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source

1
Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan
2
Faculty of Natural Sciences and Technology, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam
3
Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot 630000, Vietnam
4
Life Science Development Center, Tamkang University, New Taipei City 25137, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Arn Mignon
Polymers 2021, 13(12), 1959; https://doi.org/10.3390/polym13121959
Received: 14 May 2021 / Revised: 10 June 2021 / Accepted: 10 June 2021 / Published: 13 June 2021
(This article belongs to the Special Issue Functional Natural-Based Polymers)
Recently, there has been increasing use of agro-byproducts in microbial fermentation to produce a variety of value-added products. In this study, among various kinds of agro-byproducts, pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimal medium for sucrolytic enzyme production contained 2% PAP, 0.75% NH4NO3, 0.05% MgSO4, and 0.05% NaH2PO4 and the optimal culture conditions were pH 6.7, 35 °C, 150 rpm, and 24 h. Accordingly, the highest sucrolytic activity was 1.87 U/mL, 4.79-fold higher than that from standard conditions using sucrose as the carbon source. The purified sucrolytic enzyme (sleTKU004) is a 53 kDa monomeric protein and belongs to the glycoside hydrolase family 68. The optimum temperature and pH of sleTKU004 were 50 °C, and pH = 6, respectively. SleTKU004 could hydrolyze sucrose, raffinose, and stachyose by attacking the glycoside linkage between glucose and fructose molecules of the sucrose unit. The Km and Vmax of sleTKU004 were 1.16 M and 5.99 µmol/min, respectively. Finally, sleTKU004 showed strong sucrose tolerance and presented the highest hydrolytic activity at the sucrose concentration of 1.2 M–1.5 M. View Full-Text
Keywords: agro-byproduct; Bacillus licheniformis; bioconversion; pomelo albedo; sucrolytic agro-byproduct; Bacillus licheniformis; bioconversion; pomelo albedo; sucrolytic
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MDPI and ACS Style

Doan, C.T.; Tran, T.N.; Nguyen, T.T.; Tran, T.P.H.; Nguyen, V.B.; Tran, T.D.; Nguyen, A.D.; Wang, S.-L. Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source. Polymers 2021, 13, 1959. https://doi.org/10.3390/polym13121959

AMA Style

Doan CT, Tran TN, Nguyen TT, Tran TPH, Nguyen VB, Tran TD, Nguyen AD, Wang S-L. Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source. Polymers. 2021; 13(12):1959. https://doi.org/10.3390/polym13121959

Chicago/Turabian Style

Doan, Chien T., Thi N. Tran, Thi T. Nguyen, Thi P.H. Tran, Van B. Nguyen, Trung D. Tran, Anh D. Nguyen, and San-Lang Wang. 2021. "Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source" Polymers 13, no. 12: 1959. https://doi.org/10.3390/polym13121959

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