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Article

Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering

1
Institute of Polymer Science and Technology, ICTP-CSIC, C/Juan de la Cierva 3, 28006 Madrid, Spain
2
CIBER-BBN, Health Institute Carlos III, C/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain
3
Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, E-18100 Granada, Spain
4
Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA), University Hospitals of Granada University of Granada, E-18071 Granada, Spain
5
Department of Health Sciences, University of Jaén, 23071 Jaén, Spain
6
Excellence Research Unit “Modeling Nature” (MNat), University of Granada, E-18016 Granada, Spain
7
Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, E-18016 Granada, Spain
8
NanoBioCel Group, Laboratory of Pharmaceutics, University of the Basque Country (UPV/EHU), School of Pharmacy, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
9
R&D Human Health, Bioibérica S.A.U., 08950 Barcelona, Spain
*
Authors to whom correspondence should be addressed.
These authors contributed equally.
Polymers 2020, 12(11), 2661; https://doi.org/10.3390/polym12112661
Received: 19 October 2020 / Revised: 6 November 2020 / Accepted: 7 November 2020 / Published: 11 November 2020
(This article belongs to the Special Issue Advanced Polymers for Biomedical Applications)
In the present study, semi- and interpenetrated polymer network (IPN) systems based on hyaluronic acid (HA) and chitosan using ionic crosslinking of chitosan with a bioactive crosslinker, glycerylphytate (G1Phy), and UV irradiation of methacrylate were developed, characterized and evaluated as potential supports for tissue engineering. Semi- and IPN systems showed significant differences between them regarding composition, morphology, and mechanical properties after physicochemical characterization. Dual crosslinking process of IPN systems enhanced HA retention and mechanical properties, providing also flatter and denser surfaces in comparison to semi-IPN membranes. The biological performance was evaluated on primary human mesenchymal stem cells (hMSCs) and the systems revealed no cytotoxic effect. The excellent biocompatibility of the systems was demonstrated by large spreading areas of hMSCs on hydrogel membrane surfaces. Cell proliferation increased over time for all the systems, being significantly enhanced in the semi-IPN, which suggested that these polymeric membranes could be proposed as an effective promoter system of tissue repair. In this sense, the developed crosslinked biomimetic and biodegradable membranes can provide a stable and amenable environment for hMSCs support and growth with potential applications in the biomedical field. View Full-Text
Keywords: interpenetrated polymer network; semi-IPN; methacrylated hyaluronic acid; chitosan; glycerylphytate; mesenchymal stem cell interpenetrated polymer network; semi-IPN; methacrylated hyaluronic acid; chitosan; glycerylphytate; mesenchymal stem cell
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MDPI and ACS Style

Mora-Boza, A.; López-Ruiz, E.; López-Donaire, M.L.; Jiménez, G.; Aguilar, M.R.; Marchal, J.A.; Pedraz, J.L.; Vázquez-Lasa, B.; Román, J.S.; Gálvez-Martín, P. Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering. Polymers 2020, 12, 2661. https://doi.org/10.3390/polym12112661

AMA Style

Mora-Boza A, López-Ruiz E, López-Donaire ML, Jiménez G, Aguilar MR, Marchal JA, Pedraz JL, Vázquez-Lasa B, Román JS, Gálvez-Martín P. Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering. Polymers. 2020; 12(11):2661. https://doi.org/10.3390/polym12112661

Chicago/Turabian Style

Mora-Boza, Ana; López-Ruiz, Elena; López-Donaire, María L.; Jiménez, Gema; Aguilar, María R.; Marchal, Juan A.; Pedraz, José L.; Vázquez-Lasa, Blanca; Román, Julio S.; Gálvez-Martín, Patricia. 2020. "Evaluation of Glycerylphytate Crosslinked Semi- and Interpenetrated Polymer Membranes of Hyaluronic Acid and Chitosan for Tissue Engineering" Polymers 12, no. 11: 2661. https://doi.org/10.3390/polym12112661

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