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Production of Resveratrol by Piceid Deglycosylation Using Cellulase
Open AccessArticle

Characterization of a Metagenome-Derived β-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol

CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
Author to whom correspondence should be addressed.
Academic Editor: David D. Boehr
Catalysts 2016, 6(3), 35;
Received: 8 December 2015 / Revised: 25 January 2016 / Accepted: 19 February 2016 / Published: 1 March 2016
(This article belongs to the Special Issue Enzyme Catalysis)
PDF [1983 KB, uploaded 1 March 2016]


For the beneficial pharmacological properties of resveratrol, there is increasingly interest in enzymatic conversion of polydatin to resveratrol. The metagenomic technique provides an effective strategy for mining novel polydatin-hydrolysis enzymes from uncultured microorganisms. In this study, a metagenomic library of mangrove soil was constructed and a novel β-glucosidase gene MlBgl was isolated. The deduced amino acid sequences of MlBgl showed the highest identity of 64% with predicted β-glucosidase in the GenBank database. The gene was cloned and overexpressed in Escherichia coli BL21(DE3). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay demonstrated the purified recombinant β-glucosidase r-MlBgl with a molecular weight approximately of 71 kDa. The optimal pH and temperature of purified recombinant r-MlBgl were 7.0 and 40 °C, respectively. r-MlBgl could hydrolyze polydatin effectively. The kcat and kcat/Km values for polydatin were 989 s−1 and 1476 mM−1·s−1, respectively. These properties suggest that -r-MlBgl has potential application in the enzymatic conversion of polydatin to resveratrol for further study. View Full-Text
Keywords: β-glucosidase; mangrove soil; metagenomic library; polydatin; resveratrol β-glucosidase; mangrove soil; metagenomic library; polydatin; resveratrol

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Mai, Z.; Su, H.; Zhang, S. Characterization of a Metagenome-Derived β-Glucosidase and Its Application in Conversion of Polydatin to Resveratrol. Catalysts 2016, 6, 35.

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