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Article

Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning

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Department of Organic Chemistry and Technology, Budapest University of Technology and Economics, Műegyetem rkp. 3, 1111 Budapest, Hungary
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ELKH Research Center for Natural Sciences, Institute of Enzymology, Magyar tudósok krt. 2, 1117 Budapest, Hungary
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Department of Physical Chemistry and Materials Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, 1111 Budapest, Hungary
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Department of Electron Devices, Budapest University of Technology and Economics, Műegyetem rkp. 3, 1111 Budapest, Hungary
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SpinSplit LLC., Vend u. 17, 1025 Budapest, Hungary
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Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences, P.O. Box 286, 1519 Budapest, Hungary
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Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Műegyetem rkp. 3, 1111 Budapest, Hungary
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Biocatalysis and Biotransformation Research Centre, Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University of Cluj-Napoca, Arany János Str. 11, RO-400028 Cluj-Napoca, Romania
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Author to whom correspondence should be addressed.
Academic Editor: Wen-Yong Lou
Catalysts 2021, 11(10), 1149; https://doi.org/10.3390/catal11101149
Received: 20 August 2021 / Revised: 22 September 2021 / Accepted: 23 September 2021 / Published: 25 September 2021
(This article belongs to the Special Issue Advanced Technologies for Biocatalytic Synthesis)
Immobilization of the recombinant, plant-derived Petroselinum crispum phenylalanine ammonia lyase (PcPAL) in electrospun matrices have the potential to create promising, easy-to-use biocatalysts. Polylactic acid (PLA) a biologically inert, commercial biopolymer, was chosen as the material of the carrier system. PLA could be electrospun properly only from water-immiscible organic solvents, which limits its application as a carrier of sensitive biological objects. The emulsion electrospinning is a proper solution to overcome this issue using non-ionic emulsifiers with different hydrophilic-lipophilic balance (HLB) values. The stabilized emulsion could protect the sensitive PcPAL dissolved in the aqueous buffer phase and improve fiber formation, plus help to keep the biocatalytic activity of enzymes. In this study, the first approach is described to produce PLA nanofibers containing PcPAL enzymes by emulsion electrospinning and to use the resulted biocatalyst in the ammonia elimination reaction from l-phenylalanine. View Full-Text
Keywords: electrospinning; recombinant enzyme; enzyme entrapment; emulsion electrospinning; phenylalanine ammonia-lyase electrospinning; recombinant enzyme; enzyme entrapment; emulsion electrospinning; phenylalanine ammonia-lyase
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MDPI and ACS Style

Koplányi, G.; Sánta-Bell, E.; Molnár, Z.; Tóth, G.D.; Józó, M.; Szilágyi, A.; Ender, F.; Pukánszky, B.; Vértessy, B.G.; Poppe, L.; Balogh-Weiser, D. Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning. Catalysts 2021, 11, 1149. https://doi.org/10.3390/catal11101149

AMA Style

Koplányi G, Sánta-Bell E, Molnár Z, Tóth GD, Józó M, Szilágyi A, Ender F, Pukánszky B, Vértessy BG, Poppe L, Balogh-Weiser D. Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning. Catalysts. 2021; 11(10):1149. https://doi.org/10.3390/catal11101149

Chicago/Turabian Style

Koplányi, Gábor, Evelin Sánta-Bell, Zsófia Molnár, Gergő D. Tóth, Muriel Józó, András Szilágyi, Ferenc Ender, Béla Pukánszky, Beáta G. Vértessy, László Poppe, and Diána Balogh-Weiser. 2021. "Entrapment of Phenylalanine Ammonia-Lyase in Nanofibrous Polylactic Acid Matrices by Emulsion Electrospinning" Catalysts 11, no. 10: 1149. https://doi.org/10.3390/catal11101149

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