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Open AccessArticle

Preparation of Immobilized Lipase on Silica Clay as a Potential Biocatalyst on Synthesis of Biodiesel

1
The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China
2
Key Laboratory of Cosmetic, China National Light Industry, Beijing Technology and Business University, Beijing 100048, China
3
National Engineering Laboratory for Clean Technology of Leather Manufacture, Sichuan University, Chengdu 610065, China
*
Author to whom correspondence should be addressed.
Catalysts 2020, 10(11), 1266; https://doi.org/10.3390/catal10111266
Received: 3 October 2020 / Revised: 29 October 2020 / Accepted: 29 October 2020 / Published: 2 November 2020
Biodiesel offers an important alternative to fossil fuel. In this work, Eversa Transform 2.0 lipase was immobilized onto 3-aminopropyltriethoxysilane (APTES) modified silica clay (SC) by glutaraldehyde. The characteristics of the functionalized supports and the immobilized lipase were investigated by FTIR, TEM, BET, and XRD. The results show that the optimal conditions of lipase immobilization are as follows: 2% glutaraldehyde concentration, 15 mg/mL lipase concentration and incubating at 25 °C for 60 min. The immobilized lipase showed a high tolerance to temperature and pH variation in comparison to the free lipase. The immobilized lipase on SC was applied as a biocatalyst for the synthesis of biodiesel from methanol and canola oil. A biodiesel yield of 86% was obtained at a temperature of 45 °C via a three-step methanol addition. A conversion yield of 67% was maintained after reusing the immobilized lipase for five cycles. This work provides a strategy for the preparation of an efficient biocatalyst for the synthesis of biodiesel. View Full-Text
Keywords: lipase; silica clay; biodiesel; immobilization; APTES; glutaraldehyde lipase; silica clay; biodiesel; immobilization; APTES; glutaraldehyde
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MDPI and ACS Style

Zou, T.; Duan, Y.-d.; Wang, Q.-e; Cheng, H.-m. Preparation of Immobilized Lipase on Silica Clay as a Potential Biocatalyst on Synthesis of Biodiesel. Catalysts 2020, 10, 1266. https://doi.org/10.3390/catal10111266

AMA Style

Zou T, Duan Y-d, Wang Q-e, Cheng H-m. Preparation of Immobilized Lipase on Silica Clay as a Potential Biocatalyst on Synthesis of Biodiesel. Catalysts. 2020; 10(11):1266. https://doi.org/10.3390/catal10111266

Chicago/Turabian Style

Zou, Ting; Duan, You-dan; Wang, Qiao-e; Cheng, Hai-ming. 2020. "Preparation of Immobilized Lipase on Silica Clay as a Potential Biocatalyst on Synthesis of Biodiesel" Catalysts 10, no. 11: 1266. https://doi.org/10.3390/catal10111266

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