Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2+ γδ T Cell Alterations with Lymph Node Invasion
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Vδ2+ γδ T Cell Phenotypic Alterations Are Assessable from the Peripheral Blood of Untreated Breast Cancer Patients
2.2. Circulating Vδ2+ γδ T Cells Phenotypic Alterations Are Associated with Pathological Lymph Node Invasion in Breast Cancer Patients
2.3. High Dimensional Characterization of Vδ2+ γδ T Cell Alterations in BC Reveals Association of Senescent/Exhausted TEMRA Phenotypes with Lymph Node Invasion
3. Discussion
4. Materials and Methods
4.1. Study Participants
4.2. Clinical Samples
4.3. Mass Cytometry Staining and Data Acquisition
4.4. Data Processing and Analysis
4.4.1. Between-Group Analysis (BGA)
4.4.2. t-Distributed Stochastic Neighbor Embedding Analysis (t-SNE)
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
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Fattori, S.; Gorvel, L.; Granjeaud, S.; Rochigneux, P.; Rouvière, M.-S.; Ben Amara, A.; Boucherit, N.; Paul, M.; Dauplat, M.M.; Thomassin-Piana, J.; et al. Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2+ γδ T Cell Alterations with Lymph Node Invasion. Cancers 2021, 13, 441. https://doi.org/10.3390/cancers13030441
Fattori S, Gorvel L, Granjeaud S, Rochigneux P, Rouvière M-S, Ben Amara A, Boucherit N, Paul M, Dauplat MM, Thomassin-Piana J, et al. Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2+ γδ T Cell Alterations with Lymph Node Invasion. Cancers. 2021; 13(3):441. https://doi.org/10.3390/cancers13030441
Chicago/Turabian StyleFattori, Stéphane, Laurent Gorvel, Samuel Granjeaud, Philippe Rochigneux, Marie-Sarah Rouvière, Amira Ben Amara, Nicolas Boucherit, Magali Paul, Marie Mélanie Dauplat, Jeanne Thomassin-Piana, and et al. 2021. "Quantification of Immune Variables from Liquid Biopsy in Breast Cancer Patients Links Vδ2+ γδ T Cell Alterations with Lymph Node Invasion" Cancers 13, no. 3: 441. https://doi.org/10.3390/cancers13030441