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Article

Molecular Recognition by Silicon Nanowire Field-Effect Transistor and Single-Molecule Force Spectroscopy

Instituto de Ciencia de Materiales de Madrid, Consejo Superior de Investigaciones Cientificas, c/ Sor Juana Inés de la Cruz 3, 28049 Madrid, Spain
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Author to whom correspondence should be addressed.
Academic Editors: Amalio Fernández-Pacheco and Javier Pablo-Navarro
Micromachines 2022, 13(1), 97; https://doi.org/10.3390/mi13010097
Received: 14 December 2021 / Revised: 31 December 2021 / Accepted: 5 January 2022 / Published: 8 January 2022
Silicon nanowire (SiNW) field-effect transistors (FETs) have been developed as very sensitive and label-free biomolecular sensors. The detection principle operating in a SiNW biosensor is indirect. The biomolecules are detected by measuring the changes in the current through the transistor. Those changes are produced by the electrical field created by the biomolecule. Here, we have combined nanolithography, chemical functionalization, electrical measurements and molecular recognition methods to correlate the current measured by the SiNW transistor with the presence of specific molecular recognition events on the surface of the SiNW. Oxidation scanning probe lithography (o-SPL) was applied to fabricate sub-12 nm SiNW field-effect transistors. The devices were applied to detect very small concentrations of proteins (500 pM). Atomic force microscopy (AFM) single-molecule force spectroscopy (SMFS) experiments allowed the identification of the protein adsorption sites on the surface of the nanowire. We detected specific interactions between the biotin-functionalized AFM tip and individual avidin molecules adsorbed to the SiNW. The measurements confirmed that electrical current changes measured by the device were associated with the deposition of avidin molecules. View Full-Text
Keywords: oxidation scanning probe lithography; silicon nanowire; field-effect transistor; single-molecule force spectroscopy; AFM oxidation scanning probe lithography; silicon nanowire; field-effect transistor; single-molecule force spectroscopy; AFM
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MDPI and ACS Style

Espinosa, F.M.; Uhlig, M.R.; Garcia, R. Molecular Recognition by Silicon Nanowire Field-Effect Transistor and Single-Molecule Force Spectroscopy. Micromachines 2022, 13, 97. https://doi.org/10.3390/mi13010097

AMA Style

Espinosa FM, Uhlig MR, Garcia R. Molecular Recognition by Silicon Nanowire Field-Effect Transistor and Single-Molecule Force Spectroscopy. Micromachines. 2022; 13(1):97. https://doi.org/10.3390/mi13010097

Chicago/Turabian Style

Espinosa, Francisco M., Manuel R. Uhlig, and Ricardo Garcia. 2022. "Molecular Recognition by Silicon Nanowire Field-Effect Transistor and Single-Molecule Force Spectroscopy" Micromachines 13, no. 1: 97. https://doi.org/10.3390/mi13010097

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