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Open AccessArticle

High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces

1
School of Information and Communication Technology, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia
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Department of Engineering and System Science, Frontier Research Center on Fundamental and Applied Sciences of Matters, National Tsing Hua University, Hsinchu 30013, Taiwan
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Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
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Department of Biomechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan
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Department of Mechanical Engineering, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia
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Department of Physics, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia
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Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan
*
Authors to whom correspondence should be addressed.
Micromachines 2020, 11(3), 275; https://doi.org/10.3390/mi11030275
Received: 8 January 2020 / Revised: 2 March 2020 / Accepted: 3 March 2020 / Published: 6 March 2020
(This article belongs to the Special Issue 10th Anniversary of Micromachines)
A microfluidic chip, which can separate and enrich leukocytes from whole blood, is proposed. The chip has 10 switchback curve channels, which are connected by straight channels. The straight channels are designed to permit the inertial migration effect and to concentrate the blood cells, while the curve channels allow the Dean flow to further classify the blood cells based on the cell sizes. Hydrodynamic suction is also utilized to remove smaller blood cells (e.g., red blood cell (RBC)) in the curve channels for higher separation purity. By employing the inertial migration, Dean flow force, and hydrodynamic suction in a continuous flow system, our chip successfully separates large white blood cells (WBCs) from the whole blood with the processing rates as high as 1 × 108 cells/sec at a high recovery rate at 93.2% and very few RBCs (~0.1%). View Full-Text
Keywords: blood separation; microfluidics blood separation; microfluidics
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MDPI and ACS Style

Lombodorj, B.; Tseng, H.C.; Chang, H.-Y.; Lu, Y.-W.; Tumurpurev, N.; Lee, C.-W.; Ganbat, B.; Wu, R.-G.; Tseng, F.-G. High-Throughput White Blood Cell (Leukocyte) Enrichment from Whole Blood Using Hydrodynamic and Inertial Forces. Micromachines 2020, 11, 275.

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