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Micromachines
Volume 10
Issue 2
10.3390/mi10020132
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Article

A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells

by
Gwo-Chin Ma
1,2,
Wen-Hsiang Lin
1,
Chung-Er Huang
3,4,
Ting-Yu Chang
1,
Jia-Yun Liu
1,
Ya-Jun Yang
1,
Mei-Hui Lee
1,
Wan-Ju Wu
5,
Yun-Shiang Chang
6,* and
Ming Chen
1,5,6,7,8,9,*
1
Department of Genomic Medicine and Center for Medical Genetics, Changhua Christian Hospital; and Department of Genomic Science and Technology, Changhua Christian Hospital Healthcare System, Changhua 50046, Taiwan
2
Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan
3
International College of Semiconductor Technology, National Chiao Tung University, Hsinchu 30010, Taiwan
4
Cytoaurora Biotechnologies, Inc. Hsinchu Science Park, Hsinchu 30016, Taiwan
5
Department of Obstetrics and Gynecology, Changhua Christian Hospital, Changhua 50006, Taiwan
6
Department of Molecular Biotechnology, Da-Yeh University, Changhua 51591, Taiwan
7
Department of Obstetrics and Gynecology, College of Medicine, National Taiwan University, Taipei 10041, Taiwan
8
Department of Medical Genetics, National Taiwan University Hospital, Taipei 10041, Taiwan
9
Department of Life Science, Tunghai University, Taichung 40704, Taiwan
*
Authors to whom correspondence should be addressed.
Micromachines 2019, 10(2), 132; https://doi.org/10.3390/mi10020132
Received: 24 January 2019 / Revised: 13 February 2019 / Accepted: 14 February 2019 / Published: 17 February 2019
(This article belongs to the Special Issue Microfluidics for Cells and Other Organisms)
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Abstract

Circulating fetal cells (CFCs) in maternal blood are rare but have a strong potential to be the target for noninvasive prenatal diagnosis (NIPD). “Cell RevealTM system” is a silicon-based microfluidic platform capable to capture rare cell populations in human circulation. The platform is recently optimized to enhance the capture efficiency and system automation. In this study, spiking tests of SK-BR-3 breast cancer cells were used for the evaluation of capture efficiency. Then, peripheral bloods from 14 pregnant women whose fetuses have evidenced non-maternal genomic markers (e.g., de novo pathogenic copy number changes) were tested for the capture of circulating fetal nucleated red blood cells (fnRBCs). Captured cells were subjected to fluorescent in situ hybridization (FISH) on chip or recovered by an automated cell picker for molecular genetic analyses. The capture rate for the spiking tests is estimated as 88.1%. For the prenatal study, 2–71 fnRBCs were successfully captured from 2 mL of maternal blood in all pregnant women. The captured fnRBCs were verified to be from fetal origin. Our results demonstrated that the Cell RevealTM system has a high capture efficiency and can be used for fnRBC capture that is feasible for the genetic diagnosis of fetuses without invasive procedures. View Full-Text
Keywords: cbNIPD; fnRBC; capture efficiency; microfluidics; nanostructure cbNIPD; fnRBC; capture efficiency; microfluidics; nanostructure
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MDPI and ACS Style

Ma, G.-C.; Lin, W.-H.; Huang, C.-E.; Chang, T.-Y.; Liu, J.-Y.; Yang, Y.-J.; Lee, M.-H.; Wu, W.-J.; Chang, Y.-S.; Chen, M. A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells. Micromachines 2019, 10, 132. https://doi.org/10.3390/mi10020132

AMA Style

Ma G-C, Lin W-H, Huang C-E, Chang T-Y, Liu J-Y, Yang Y-J, Lee M-H, Wu W-J, Chang Y-S, Chen M. A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells. Micromachines. 2019; 10(2):132. https://doi.org/10.3390/mi10020132

Chicago/Turabian Style

Ma, Gwo-Chin, Wen-Hsiang Lin, Chung-Er Huang, Ting-Yu Chang, Jia-Yun Liu, Ya-Jun Yang, Mei-Hui Lee, Wan-Ju Wu, Yun-Shiang Chang, and Ming Chen. 2019. "A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells" Micromachines 10, no. 2: 132. https://doi.org/10.3390/mi10020132

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