The Effects of Varying Combinations of Dietary Selenium, Vitamin E, and Zinc Supplements on Antioxidant Enzyme Activity, and Developmental and Histological Traits in Testicular Tissues of 1-Year-Old Native Turkish Ganders

Round 1

Reviewer 1 Report

The authors have determined the effects of dietary Selenium, Vitamin E , Zinc and their varying combinations supplementation on oxidative status, developmental and histological traits in testicular tissue of 1-year-old native Turkish ganders. This study has found that dietary supplementation of Se+Vit E+Zn desplayed the positive effects on native Turkish ganders on the testicular tissue, reducing oxidative stress and improving histological parameters without affecting their physiological status. The experimental was well-desgisned and the data was well-presented. The following revision could improve the quality of the paper.

1. Did you analyzed the sperm quality data? Why not added it? 

2. L125, Table, Were these data (the cocentration of the Se, Vit E, and Zn in the basle diet) analzyed? These data are very important.

3. L201, All the P value needs to be writted in italic. Please check throughout thet paper. 

4. Please add the replicates n=? in the figure lengeds. Such as the figuer 2. 

5. Please explained the doses of Se, Vit E, and Zn have been used in the study.

6. Please add some latest references about the effects of Se, Vit E, and Zn on their roles in the male reproductive system. Such as: Selenium deficiency-induced multiple tissue damage with dysregulation of immune and redox homeostasis in broiler chicks under heat stress, Science China Life Sciences, 2023; Both selenium deficiency and excess impair male reproductive system via inducing oxidative stress-activated PI3K/AKT-mediated apoptosis and cell proliferation signaling in testis of mice, Free Radical Biology and Medicine, 2023.  

7. Please check the references and make sure they are following the journal style. 

8. Please increase the resolution of the Figures. 

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

The authors mentioned lipid peroxidation as the story in the Introduction, but there was no lipid oxidation product, i.e., the oxidized lipid molecules, tested in the work. If such data could be added, the story would be more complete.

The author did not set modeling groups, and I have no idea if the work specifically aimed at oxidative stress.

Please the authors describe the reason for the amount of Se, Zn, and Vitamin E in the experiment. Moreover, according
design and grouping, dose-dependent manner would not be observed, which could help enhance the data.

Since the authors tested SOD, CAT, GPx, GST, and MDA, may the authors speculate on the possible pathway of antioxidation effects?

The authors conducted correlation analysis, and I wonder why did the authors do it. Please connect the findings to the theme of the manuscript.

Sperm production and activity relate to not only oxidative stress but also other factors, for example, mitochondria function. Please discuss the specificity of this work aiming to "antioxidation" to fit the journal scope.

There seemed not so many significant English writing problems.

Author Response

Please see the attachment.

Author Response File: Author Response.docx

Round 2

Reviewer 2 Report

The authors did proper modifications. Now the manuscript quality has been improved.

Almost fine.