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Article

Genetic Variation Analysis of Superior Cotton Varieties of Gossypium hirsutum through Microsatellite Markers

by
Dede Nuraida
1,*,
Yusuf Abdurrajak
2,
Moh Amin
2 and
Utami S. Hastutik
2
1
University of PGRI Ronggolawe, Jalan Manunggal No. 61, Gedongombo, Semanding, Gedongombo, Semanding, Kabupaten Tuban, Jawa Timur 3523120, Indonesia
2
State University of Malang, East Java 65145, Indonesia
*
Author to whom correspondence should be addressed.
Int. J. Plant Biol. 2017, 8(1), 6996; https://doi.org/10.4081/pb.2017.6996
Submission received: 2 December 2016 / Revised: 1 February 2017 / Accepted: 1 February 2017 / Published: 12 April 2017

Abstract

This study was conducted in order to obtain information on genetic variation in populations rated as superior cotton (Gossypium hirsutum L.) varieties in Balittas Malang, Indonesia. The samples used 10 varieties of cotton Kanesia series and 2 other superior varieties that are LRA 5166 and ISA 205A. Indicators of genetic diversity are the number of alleles per locus, allele frequencies, and heterozygosity values. DNA was isolated from the leaves of 3- week-old seedlings using the CTAB method. Amplification was performed using 5 SSRs primer pairs of the JESPR series. The results showed five microsatellite loci, yielding 12 alleles with a size range of 80–500 bp, with an average number of alleles per locus of 4.60. The average values of heterozygosity of the five loci was high, at 0.71. Based on the number of alleles, allele frequencies and heterozygosity values, the genetic variation sampled in the superior cotton varieties studied here is quite high.
Keywords: cotton superior; genetic variation; allele; heterozygosity cotton superior; genetic variation; allele; heterozygosity

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MDPI and ACS Style

Nuraida, D.; Abdurrajak, Y.; Amin, M.; Hastutik, U.S. Genetic Variation Analysis of Superior Cotton Varieties of Gossypium hirsutum through Microsatellite Markers. Int. J. Plant Biol. 2017, 8, 6996. https://doi.org/10.4081/pb.2017.6996

AMA Style

Nuraida D, Abdurrajak Y, Amin M, Hastutik US. Genetic Variation Analysis of Superior Cotton Varieties of Gossypium hirsutum through Microsatellite Markers. International Journal of Plant Biology. 2017; 8(1):6996. https://doi.org/10.4081/pb.2017.6996

Chicago/Turabian Style

Nuraida, Dede, Yusuf Abdurrajak, Moh Amin, and Utami S. Hastutik. 2017. "Genetic Variation Analysis of Superior Cotton Varieties of Gossypium hirsutum through Microsatellite Markers" International Journal of Plant Biology 8, no. 1: 6996. https://doi.org/10.4081/pb.2017.6996

APA Style

Nuraida, D., Abdurrajak, Y., Amin, M., & Hastutik, U. S. (2017). Genetic Variation Analysis of Superior Cotton Varieties of Gossypium hirsutum through Microsatellite Markers. International Journal of Plant Biology, 8(1), 6996. https://doi.org/10.4081/pb.2017.6996

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