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Article

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine

by 1, 1,2,*, 1, 1, 1, 1 and 1,3,*
1
Department of Pharmaceutical Analysis, School of Pharmacy, Xi’an Jiaotong University, Xi’an 710061, China
2
Department of Pharmaceutical Analysis, School of Pharmacy, Key Laboratory of Ningxia Ethnomedicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750004, China
3
Department of Pharmaceutical Analysis, College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Juan José Torrado
Pharmaceutics 2022, 14(6), 1154; https://doi.org/10.3390/pharmaceutics14061154
Received: 13 April 2022 / Revised: 21 May 2022 / Accepted: 25 May 2022 / Published: 28 May 2022
Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system. The encapsulated enzyme was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and a vibrating sample magnetometer. Finally, a colorimetric sensing method was developed for the detection of glucose in urine samples based on the encapsulated glucose oxidase and a hydrogen peroxide test strip. The method exhibited a good linear performance in the concentration range of 20~160 μg mL−1 and good recoveries ranging from 94.3 to 118.0%. This work proves that the self-assembly method could be employed to encapsulate glucose oxidase into silica-coated magnetic particles. The developed colorimetric sensing method shows high sensitivity, which will provide a promising tool for the detection of glucose and the monitoring of diabetes. View Full-Text
Keywords: enzyme encapsulation; glucose oxidase; colorimetric sensing method; glucose enzyme encapsulation; glucose oxidase; colorimetric sensing method; glucose
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MDPI and ACS Style

Luo, Z.; Chen, G.; Yang, K.; Wang, L.; Cui, X.; Xu, J.; Fu, Q. Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine. Pharmaceutics 2022, 14, 1154. https://doi.org/10.3390/pharmaceutics14061154

AMA Style

Luo Z, Chen G, Yang K, Wang L, Cui X, Xu J, Fu Q. Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine. Pharmaceutics. 2022; 14(6):1154. https://doi.org/10.3390/pharmaceutics14061154

Chicago/Turabian Style

Luo, Zhimin, Guoning Chen, Ke Yang, Lu Wang, Xia Cui, Jiameng Xu, and Qiang Fu. 2022. "Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine" Pharmaceutics 14, no. 6: 1154. https://doi.org/10.3390/pharmaceutics14061154

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