Biological Evaluation of the Effect of Root Canal Sealers Using a Rat Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethical Statement
2.2. Root Canal Sealer Components and Material Preparation
2.3. Subcutaneous Implantation Procedure
2.4. Histological Evaluation of the Tissue around the Root Canal Sealer
2.5. Microstructure Analysis Using Scanning Electron Microscopy (SEM)
2.6. Elemental Analysis of the Subcutaneous Tissue Affected by the Root Canal Sealer
2.7. RNA Extraction, RNA Sequencing (RNA-seq) and Data Analysis
2.8. Gene Ontology Analysis
3. Results
3.1. Histopathological Evaluation of the Tissue around the Root Canal Sealer
3.2. Microstructure Analysis Using SEM
3.3. Elemental Analysis of the Subcutaneous Tissue Affected by the Root Canal Sealer
3.4. Transcriptome and Gene Ontology Enrichment Analysis of Differentially Expressed Genes in the Subcutaneous Tissues
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Materials | Main Composition | |
---|---|---|
Canal sealer N zinc oxide-fatty acid sealer | Fatty Acid based Zinc Oxide Sealer (Nippon Shika Yakuhin Co., Ltd.) | olive oil, zinc oxide, bismuth hypocarbonate, magnesium oxide, fatty acid, rosin, ester gum. Lot number J7S |
Metaseal soft paste methacrylate resin sealer | Resin-based root canal sealer (MetaSEAL Soft Paste, SUN MEDICAL) | methacrylic esters (HEMA, 4-META, others), water, photopolymerization initiator, contrast agent, organic filler. Lot number VRA |
Canal sealer BG Bio-glass contained sealer | Fatty Acid based Zinc Oxide Sealer containing Bioactive Glass (Nippon Shika Yakuhin Co., Ltd.) | magnesium oxide, purified water, calcium silicate glass, silicon dioxide, fatty acids, bismuth hypocarbonate. Lot number K3K |
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Share and Cite
Okamoto, M.; Matsumoto, S.; Moriyama, K.; Huang, H.; Watanabe, M.; Miura, J.; Sugiyama, K.; Hirose, Y.; Mizuhira, M.; Kuriki, N.; et al. Biological Evaluation of the Effect of Root Canal Sealers Using a Rat Model. Pharmaceutics 2022, 14, 2038. https://doi.org/10.3390/pharmaceutics14102038
Okamoto M, Matsumoto S, Moriyama K, Huang H, Watanabe M, Miura J, Sugiyama K, Hirose Y, Mizuhira M, Kuriki N, et al. Biological Evaluation of the Effect of Root Canal Sealers Using a Rat Model. Pharmaceutics. 2022; 14(10):2038. https://doi.org/10.3390/pharmaceutics14102038
Chicago/Turabian StyleOkamoto, Motoki, Sayako Matsumoto, Kiichi Moriyama, Hailing Huang, Masakatsu Watanabe, Jiro Miura, Keita Sugiyama, Yujiro Hirose, Manabu Mizuhira, Nanako Kuriki, and et al. 2022. "Biological Evaluation of the Effect of Root Canal Sealers Using a Rat Model" Pharmaceutics 14, no. 10: 2038. https://doi.org/10.3390/pharmaceutics14102038