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Article

Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

1
Institute of Experimental Medicine, Acad. Pavlov Street 12, 197376 St. Petersburg, Russia
2
Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy pr. 31, 199004 St. Petersburg, Russia
3
Institute of Chemistry, Saint-Petersburg State University, Universitetsky pr. 26, 198504 St. Petersburg, Russia
*
Authors to whom correspondence should be addressed.
Academic Editor: Avi Domb
Pharmaceutics 2021, 13(5), 672; https://doi.org/10.3390/pharmaceutics13050672
Received: 14 April 2021 / Revised: 3 May 2021 / Accepted: 4 May 2021 / Published: 7 May 2021
(This article belongs to the Special Issue Advances in Delivering Protein and Peptide Therapeutics)
Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro- and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro- and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro- and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods. View Full-Text
Keywords: recombinant CD81-streptavidin fusion protein; trapping system for HCV; poly(lactic acid)-based micro- and nanoparticles; interaction of LEL CD81 with E2 protein; virus-mimicking particles recombinant CD81-streptavidin fusion protein; trapping system for HCV; poly(lactic acid)-based micro- and nanoparticles; interaction of LEL CD81 with E2 protein; virus-mimicking particles
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MDPI and ACS Style

Polyakov, D.; Sinitsyna, E.; Grudinina, N.; Antipchik, M.; Sakhabeev, R.; Korzhikov-Vlakh, V.; Shavlovsky, M.; Korzhikova-Vlakh, E.; Tennikova, T. Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus. Pharmaceutics 2021, 13, 672. https://doi.org/10.3390/pharmaceutics13050672

AMA Style

Polyakov D, Sinitsyna E, Grudinina N, Antipchik M, Sakhabeev R, Korzhikov-Vlakh V, Shavlovsky M, Korzhikova-Vlakh E, Tennikova T. Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus. Pharmaceutics. 2021; 13(5):672. https://doi.org/10.3390/pharmaceutics13050672

Chicago/Turabian Style

Polyakov, Dmitry, Ekaterina Sinitsyna, Natalia Grudinina, Mariia Antipchik, Rodion Sakhabeev, Viktor Korzhikov-Vlakh, Mikhail Shavlovsky, Evgenia Korzhikova-Vlakh, and Tatiana Tennikova. 2021. "Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus" Pharmaceutics 13, no. 5: 672. https://doi.org/10.3390/pharmaceutics13050672

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