Viruses 2015, 7(6), 3329-3344; https://doi.org/10.3390/v7062774
Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry
1
ANSES, Sophia-Antipolis Laboratory, Bee Diseases Unit, BP 111, 06902 Sophia Antipolis, France
2
Aix-Marseille Université, CNRS, AFMB UMR 7257, 13288 Marseille, France
3
Institute of Molecular and Cellular Pharmacology, IPMC, UMR6097 CNRS, 660 route des Lucioles, 06560 Valbonne, France
†
Deceased.
*
Author to whom correspondence should be addressed.
Academic Editors: Elke Genersch and Sebastian Gisder
Received: 25 March 2015 / Revised: 5 June 2015 / Accepted: 15 June 2015 / Published: 23 June 2015
(This article belongs to the Special Issue Honeybee Viruses)
Abstract
Chronic bee paralysis virus (CBPV) is the etiological agent of chronic paralysis, an infectious and contagious disease in adult honeybees. CBPV is a positive single-stranded RNA virus which contains two major viral RNA fragments. RNA 1 (3674 nt) and RNA 2 (2305 nt) encode three and four putative open reading frames (ORFs), respectively. RNA 1 is thought to encode the viral RNA-dependent RNA polymerase (RdRp) since the amino acid sequence derived from ORF 3 shares similarities with the RdRP of families Nodaviridae and Tombusviridae. The genomic organization of CBPV and in silico analyses have suggested that RNA 1 encodes non-structural proteins, while RNA 2 encodes structural proteins, which are probably encoded by ORFs 2 and 3. In this study, purified CBPV particles were used to characterize virion proteins by mass spectrometry. Several polypeptides corresponding to proteins encoded by ORF 2 and 3 on RNA 2 were detected. Their role in the formation of the viral capsid is discussed. View Full-TextKeywords:
chronic bee paralysis virus (CBPV); nano-HPLC; MALDI-TOF/TOF; protein identification; hypothetical structural protein (hSP); predicted structural protein (pSP); anti-pSP antibodies
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Chevin, A.; Coutard, B.; Blanchard, P.; Dabert-Gay, A.-S.; Ribière-Chabert, M.; Thiéry, R. Characterisation of Structural Proteins from Chronic Bee Paralysis Virus (CBPV) Using Mass Spectrometry. Viruses 2015, 7, 3329-3344.