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Viruses 2015, 7(5), 2727-2744;

Characterization of Novel Transcripts in Pseudorabies Virus

Department of Medical Biology, Faculty of Medicine, University of Szeged, Somogyi B. u. 4., Szeged H-6720, Hungary
Agricultural Biotechnology Center, Institute for Plant Biotechnology, Plant Developmental Biology Group, Szent-Györgyi A. u. 4, Gödöllő H-2100, Hungary
Department of Genetics, School of Medicine, Stanford University, 300 Pasteur Dr., Stanford, CA 94305-5120, USA
These authors contributed equally to this work.
These two authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Eric O. Freed
Received: 15 April 2015 / Revised: 14 May 2015 / Accepted: 18 May 2015 / Published: 22 May 2015
(This article belongs to the Section Animal Viruses)
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In this study we identified two 3′-coterminal RNA molecules in the pseudorabies virus. The highly abundant short transcript (CTO-S) proved to be encoded between the ul21 and ul22 genes in close vicinity of the replication origin (OriL) of the virus. The less abundant long RNA molecule (CTO-L) is a transcriptional readthrough product of the ul21 gene and overlaps OriL. These polyadenylated RNAs were characterized by ascertaining their nucleotide sequences with the Illumina HiScanSQ and Pacific Biosciences Real-Time (PacBio RSII) sequencing platforms and by analyzing their transcription kinetics through use of multi-time-point Real-Time RT-PCR and the PacBio RSII system. It emerged that transcription of the CTOs is fully dependent on the viral transactivator protein IE180 and CTO-S is not a microRNA precursor. We propose an interaction between the transcription and replication machineries at this genomic location, which might play an important role in the regulation of DNA synthesis. View Full-Text
Keywords: non-coding RNA; pseudorabies virus; herpesvirus; RNA sequencing; DNA replication non-coding RNA; pseudorabies virus; herpesvirus; RNA sequencing; DNA replication

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Tombácz, D.; Csabai, Z.; Oláh, P.; Havelda, Z.; Sharon, D.; Snyder, M.; Boldogkői, Z. Characterization of Novel Transcripts in Pseudorabies Virus. Viruses 2015, 7, 2727-2744.

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