Characterization of Novel Transcripts in Pseudorabies Virus
AbstractIn this study we identified two 3′-coterminal RNA molecules in the pseudorabies virus. The highly abundant short transcript (CTO-S) proved to be encoded between the ul21 and ul22 genes in close vicinity of the replication origin (OriL) of the virus. The less abundant long RNA molecule (CTO-L) is a transcriptional readthrough product of the ul21 gene and overlaps OriL. These polyadenylated RNAs were characterized by ascertaining their nucleotide sequences with the Illumina HiScanSQ and Pacific Biosciences Real-Time (PacBio RSII) sequencing platforms and by analyzing their transcription kinetics through use of multi-time-point Real-Time RT-PCR and the PacBio RSII system. It emerged that transcription of the CTOs is fully dependent on the viral transactivator protein IE180 and CTO-S is not a microRNA precursor. We propose an interaction between the transcription and replication machineries at this genomic location, which might play an important role in the regulation of DNA synthesis. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 228 KB)
Share & Cite This Article
Tombácz, D.; Csabai, Z.; Oláh, P.; Havelda, Z.; Sharon, D.; Snyder, M.; Boldogkői, Z. Characterization of Novel Transcripts in Pseudorabies Virus. Viruses 2015, 7, 2727-2744.
Tombácz D, Csabai Z, Oláh P, Havelda Z, Sharon D, Snyder M, Boldogkői Z. Characterization of Novel Transcripts in Pseudorabies Virus. Viruses. 2015; 7(5):2727-2744.Chicago/Turabian Style
Tombácz, Dóra; Csabai, Zsolt; Oláh, Péter; Havelda, Zoltán; Sharon, Donald; Snyder, Michael; Boldogkői, Zsolt. 2015. "Characterization of Novel Transcripts in Pseudorabies Virus." Viruses 7, no. 5: 2727-2744.