Next Article in Journal
Retrospective Serology Study of Respiratory Virus Infections in Captive Great Apes
Previous Article in Journal
Single-Dose Intranasal Treatment with DEF201 (Adenovirus Vectored Consensus Interferon) Prevents Lethal Disease Due to Rift Valley Fever Virus Challenge
Article Menu

Export Article

Open AccessArticle
Viruses 2014, 6(3), 1424-1441; https://doi.org/10.3390/v6031424

IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts

1
Maryland Pathogen Research Institute, College Park, MD 20742, USA
2
Department of Cell Biology and Molecular Genetics University of Maryland, College Park, MD 20742, USA
*
Author to whom correspondence should be addressed.
Received: 22 January 2014 / Revised: 5 March 2014 / Accepted: 7 March 2014 / Published: 24 March 2014
(This article belongs to the Section Animal Viruses)
Full-Text   |   PDF [1027 KB, uploaded 12 May 2015]   |  

Abstract

Although dermal fibroblasts are one of the first cell types exposed to West Nile virus (WNV) during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, we demonstrate that neuroinvasive, WNV-New York (WNV-NY), and nonneuroinvasive, WNV-Australia (WNV-AUS60) strains are able to infect and replicate in primary human dermal fibroblasts (HDFs). However, WNV-AUS60 replication and spread within HDFs was reduced compared to that of WNV-NY due to an interferon (IFN)-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-β-dependent reduction in particle infectivity. Overall, our data indicates that human dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HDFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir in vivo. View Full-Text
Keywords: West Nile virus; interferon; human dermal fibroblast; antiviral response; intracellular innate immunity West Nile virus; interferon; human dermal fibroblast; antiviral response; intracellular innate immunity
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Hoover, L.I.; Fredericksen, B.L. IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal Fibroblasts. Viruses 2014, 6, 1424-1441.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top