Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections
Abstract
:1. Introduction
2. Results and Discussion
2.1. Prop5 Protected Animals from Influenza Virus Infections
Pretreatment | No. of survives/total no. | MSD, (days) | Lung index (%) | Virus titre (Log10 TCID50/g lung ± SD) | ||||
---|---|---|---|---|---|---|---|---|
Day 2 | Day 4 | Day 6 | Day 2 | Day 4 | Day 6 | |||
Prop5 (5 mg/kg/d) | 8/10 | 12.6 ± 3.13 * | 0.79 ± 0.06 | 1.22 ± 0.64 | 1.15 ± 0.21 | 3.31 ± 0.09 | 3.03 ± 0.17 ** | 2.91 ± 0.52 ** |
Prop5 (10 mg/kg/d) | 8/10 | 13.6± 0.89 ** | 0.83 ± 0.03 | 0.87 ± 0.17 | 0.98 ± 0.03 | 2.26 ± 0.02 * | 2.81 ± 0.31 ** | 2.72 ± 0.44 ** |
Prop5 (20 mg/kg/d) | 10/10 | 14.0 ** | 0.92 ± 0.07 | 0.95 ± 0.14 | 0.81 ± 0.07 * | 1.41 ± 0.35 ** | 2.66 ± 0.27 ** | 2.40 ± 0.13 ** |
Prop5R (20 mg/kg/d) | 0/10 | 6.6 ± 0.54 | 0.75 ± 0.05 | 1.17 ± 0.68 | 1.31 ± 0.43 | 3.48 ± 0.10 | 4.27 ± 1.19 | 4.92 ± 0.99 |
Infected control | 0/10 | 7.4 ± 1.51 | 0.75 ± 0.08 | 1.38 ± 0.98 | 1.27 ± 0.24 | 4.12 ± 0.07 | 5.45 ± 0.50 | 5.74 ± 1.14 |
Uninfected control | 10/10 | 14.0 | 0.67 ± 0.05 | 0.82 ± 0.06 | 0.80 ± 0.09 | NA | NA | NA |
2.2. Prop5 Decreased the Lung Infection Parameters
2.3. Reduction of PDCD5 Protein Expression Levels by prop5 in BALB/c Mice
3. Experimental Section
3.1. Virus, Cell, Mice, and ASODNs
3.2. Detecting Expression of PDCD5 Protein by Western Blotting and Immunohistochemical Staining
3.4. Lung Infection Parameters
3.5. Statistics Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References and Notes
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Li, K.; Zhou, Z.; Wang, Y.O.; Liu, J.; Zhao, H.B.; Yang, J.; Wang, S.Q. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses 2014, 6, 573-581. https://doi.org/10.3390/v6020573
Li K, Zhou Z, Wang YO, Liu J, Zhao HB, Yang J, Wang SQ. Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections. Viruses. 2014; 6(2):573-581. https://doi.org/10.3390/v6020573
Chicago/Turabian StyleLi, Kang, Zhe Zhou, Yu Ou Wang, Juan Liu, Hai Bao Zhao, Jing Yang, and Sheng Qi Wang. 2014. "Pretreatment of Mice with Oligonucleotide prop5 Protects Them from Influenza Virus Infections" Viruses 6, no. 2: 573-581. https://doi.org/10.3390/v6020573