Review Reports - Should SVGp12 Be Used for JC Polyomavirus Studies? Comment on Prezioso et al. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains. <i>Viruses</i> 2022, <i>14</i>, 2070

Round 1

Reviewer 1 Report

The comment draws attention to an article published eight years ago. A productive
BKPyV infection was unexpectedly found in the SVGp12 cell line. Its use to
study the infectious cycle of the closely related JCPyV virus is problematic.

Reviewer 2 Report

In this letter, the commenters point out the material (cell line) used in the report published in Viruses by Prezioso et al. As they note, in the original publication, SVG p12 was implemented in the in vitro experiments of JC polyomavirus. This cell line is known to be persistently infected with the BK polyomavirus, which is closely related to JCV. The commenters' advice is essential for this paper and for a wide range of studies using SVG p12 cells. Prezioso et al. obtained similar results using cell lines other than SVG p12, which may not affect the conclusions in the published report. However, this reviewer suggests that they need to explain carefully the points made by the commenters.