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Article

Immunization with Virus-Like Particle Vaccine Protects Rabbits against Hepatitis E-3 Virus Infection

1
Department of Infectious Disease, College of Veterinary Medicine, Konkuk University, 120 Neundong-ro, Gwangjin-gu, Seoul 05029, Korea
2
KU Center for Animal Blood Medical Science, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
3
Konkuk University Zoonotic Diseases Research Center, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
4
Department of Laboratory Animal Medicine, College of Veterinary Medicine, Konkuk University, 120 Neundong-ro, Gwangjin-gu, Seoul 05029, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Huichen Guo and Sahibzada Waheed Abdullah
Viruses 2022, 14(7), 1432; https://doi.org/10.3390/v14071432
Received: 26 May 2022 / Revised: 25 June 2022 / Accepted: 27 June 2022 / Published: 29 June 2022
(This article belongs to the Special Issue Virus-Like Particle Vaccines 2022)
Here, rabbits were immunized with a virus-like particle (VLP) vaccine prepared by expressing 239 amino acids of the swine hepatitis E virus (HEV)-3 capsid protein using a baculovirus system. Thirty specific-pathogen-free rabbits were divided into five groups (negative and positive control and 10, 50, and 100 μg VLP-vaccinated). Positive control group rabbits showed viremia and fecal viral shedding, whereas rabbits vaccinated with 10 μg VLP showed transient fecal viral shedding, and rabbits vaccinated with 50 and 100 μg VLP did not show viremia or fecal viral shedding. Serum anti-HEV antibody titers increased in a dose-dependent manner. Anti-HEV antibody titers were significantly higher (p < 0.05) in 100 μg VLP-vaccinated rabbits than in the negative control rabbits at week 4. Anti-HEV antibody titers were significantly higher in 50 and 10 μg VLP-vaccinated rabbits than in the negative control rabbits at weeks 8 and 11, respectively. Serum IFN-γ and IL-12 levels were significantly higher (p < 0.01) in rabbits vaccinated with 50 and 100 μg VLP than in the negative control rabbits at weeks 4 and 6. Liver tissues of 50 and 100 μg VLP-vaccinated rabbits displayed significantly less (p < 0.05) fibrosis than those of the positive control rabbits. The prepared VLP vaccine demonstrated dose-dependent immunogenicity sufficient for inducing anti-HEV antibody production, thus protecting rabbits against swine HEV-3. View Full-Text
Keywords: hepatitis E virus; virus-like particle; rabbit; antibody; liver fibrosis; cytokine hepatitis E virus; virus-like particle; rabbit; antibody; liver fibrosis; cytokine
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MDPI and ACS Style

Go, H.-J.; Park, B.-J.; Ahn, H.-S.; Han, S.-H.; Kim, D.-H.; Lyoo, E.-L.; Kim, D.-Y.; Kim, J.-H.; Lee, J.-B.; Park, S.-Y.; Song, C.-S.; Lee, S.-W.; Choi, Y.-K.; Choi, I.-S. Immunization with Virus-Like Particle Vaccine Protects Rabbits against Hepatitis E-3 Virus Infection. Viruses 2022, 14, 1432. https://doi.org/10.3390/v14071432

AMA Style

Go H-J, Park B-J, Ahn H-S, Han S-H, Kim D-H, Lyoo E-L, Kim D-Y, Kim J-H, Lee J-B, Park S-Y, Song C-S, Lee S-W, Choi Y-K, Choi I-S. Immunization with Virus-Like Particle Vaccine Protects Rabbits against Hepatitis E-3 Virus Infection. Viruses. 2022; 14(7):1432. https://doi.org/10.3390/v14071432

Chicago/Turabian Style

Go, Hyeon-Jeong, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Dong-Hwi Kim, Eu-Lim Lyoo, Da-Yoon Kim, Jae-Hyeong Kim, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Yang-Kyu Choi, and In-Soo Choi. 2022. "Immunization with Virus-Like Particle Vaccine Protects Rabbits against Hepatitis E-3 Virus Infection" Viruses 14, no. 7: 1432. https://doi.org/10.3390/v14071432

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