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Review

Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests

1
ZIP Diagnostics Pty Ltd., Collingwood, VIC 3066, Australia
2
Department of Medicine, University of Melbourne, Melbourne, VIC 3010, Australia
3
Department of Infectious Diseases, Monash University, Melbourne, VIC 3004, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Daniele Focosi
Viruses 2022, 14(6), 1311; https://doi.org/10.3390/v14061311
Received: 23 May 2022 / Revised: 12 June 2022 / Accepted: 13 June 2022 / Published: 15 June 2022
The COVID-19 pandemic has resulted in an unprecedented global demand for in vitro diagnostic reagents. Supply shortages and hoarding have impacted testing capacity which has led to inefficient COVID-19 case identification and transmission control, predominantly in developing countries. Traditionally, RNA extraction is a prerequisite for conducting SARS-CoV-2 nucleic acid amplification tests (NAAT); however, simplified methods of sample processing have been successful at bypassing typical nucleic acid extraction steps, enabling extraction-free SARS-CoV-2 NAAT workflows. These methods involve chemical and physical approaches that are inexpensive and easily accessible alternatives to overcome extraction kit supply shortages, while offering acceptable test performance. Here we provide an overview of three main sample preparation strategies that have been shown to facilitate extraction-free SARS-CoV-2 NAATs. View Full-Text
Keywords: COVID-19; SARS-CoV-2; nucleic acid extraction; diagnostics; NAAT; isothermal amplification; RT-PCR COVID-19; SARS-CoV-2; nucleic acid extraction; diagnostics; NAAT; isothermal amplification; RT-PCR
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MDPI and ACS Style

Delgado-Diaz, D.J.; Sakthivel, D.; Nguyen, H.H.T.; Farrokzhad, K.; Hopper, W.; Narh, C.A.; Richards, J.S. Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests. Viruses 2022, 14, 1311. https://doi.org/10.3390/v14061311

AMA Style

Delgado-Diaz DJ, Sakthivel D, Nguyen HHT, Farrokzhad K, Hopper W, Narh CA, Richards JS. Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests. Viruses. 2022; 14(6):1311. https://doi.org/10.3390/v14061311

Chicago/Turabian Style

Delgado-Diaz, David J., Dhanasekaran Sakthivel, Hanh H. T. Nguyen, Khashayar Farrokzhad, William Hopper, Charles A. Narh, and Jack S. Richards. 2022. "Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests" Viruses 14, no. 6: 1311. https://doi.org/10.3390/v14061311

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