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Article

Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features

1
Department of Biochemistry and Molecular Biology, Center for Structural Biology, McKnight Brain Institute, College of Medicine, University of Florida, Gainesville, FL 32610, USA
2
Biological Science Imaging Resource, Department of Biological Sciences, Florida State University, Tallahassee, FL 32306, USA
*
Author to whom correspondence should be addressed.
Current address: Shared Materials Instrumentation Facility, 101 Science Dr, Duke University, Durham, NC 27708, USA.
Academic Editor: Giorgio Gallinella
Viruses 2021, 13(1), 101; https://doi.org/10.3390/v13010101
Received: 22 December 2020 / Accepted: 7 January 2021 / Published: 13 January 2021
(This article belongs to the Special Issue Advances in Parvovirus Research 2020)
The capsid structures of most Adeno-associated virus (AAV) serotypes, already assigned to an antigenic clade, have been previously determined. This study reports the remaining capsid structures of AAV7, AAV11, AAV12, and AAV13 determined by cryo-electron microscopy and three-dimensional image reconstruction to 2.96, 2.86, 2.54, and 2.76 Å resolution, respectively. These structures complete the structural atlas of the AAV serotype capsids. AAV7 represents the first clade D capsid structure; AAV11 and AAV12 are of a currently unassigned clade that would include AAV4; and AAV13 represents the first AAV2-AAV3 hybrid clade C capsid structure. These newly determined capsid structures all exhibit the AAV capsid features including 5-fold channels, 3-fold protrusions, 2-fold depressions, and a nucleotide binding pocket with an ordered nucleotide in genome-containing capsids. However, these structures have viral proteins that display clade-specific loop conformations. This structural characterization completes our three-dimensional library of the current AAV serotypes to provide an atlas of surface loop configurations compatible with capsid assembly and amenable for future vector engineering efforts. Derived vectors could improve gene delivery success with respect to specific tissue targeting, transduction efficiency, antigenicity or receptor retargeting. View Full-Text
Keywords: AAV; serotype; capsid; cryo-EM; genome packaging; gene delivery AAV; serotype; capsid; cryo-EM; genome packaging; gene delivery
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MDPI and ACS Style

Mietzsch, M.; Jose, A.; Chipman, P.; Bhattacharya, N.; Daneshparvar, N.; McKenna, R.; Agbandje-McKenna, M. Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features. Viruses 2021, 13, 101. https://doi.org/10.3390/v13010101

AMA Style

Mietzsch M, Jose A, Chipman P, Bhattacharya N, Daneshparvar N, McKenna R, Agbandje-McKenna M. Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features. Viruses. 2021; 13(1):101. https://doi.org/10.3390/v13010101

Chicago/Turabian Style

Mietzsch, Mario, Ariana Jose, Paul Chipman, Nilakshee Bhattacharya, Nadia Daneshparvar, Robert McKenna, and Mavis Agbandje-McKenna. 2021. "Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features" Viruses 13, no. 1: 101. https://doi.org/10.3390/v13010101

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