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Open AccessArticle

A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia

1
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, WI 53705, USA
2
Department of Surgery, University of Wisconsin, Madison, WI 53705, USA
3
Department of Biomedical Engineering, University of Wisconsin, Madison, WI 53705, USA
4
Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI 53705, USA
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(4), 450; https://doi.org/10.3390/v12040450
Received: 13 February 2020 / Revised: 3 April 2020 / Accepted: 11 April 2020 / Published: 16 April 2020
(This article belongs to the Section Animal Viruses)
Human head and neck cancers that develop from the squamous cells of the oropharynx (Oropharyngeal Squamous Cell Carcinomas or OPSCC) are commonly associated with the papillomavirus infection. A papillomavirus infection-based mouse model of oropharyngeal tumorigenesis would be valuable for studying the development and treatment of these tumors. We have developed an efficient system using the mouse papillomavirus (MmuPV1) to generate dysplastic oropharyngeal lesions, including tumors, in the soft palate and the base of the tongue of two immune-deficient strains of mice. To maximize efficiency and safety during infection and endoscopy, we have designed a nose cone for isoflurane-induced anesthesia that takes advantage of a mouse’s need to breathe nasally and has a large window for oral manipulations. To reach and infect the oropharynx efficiently, we have repurposed the Greer Pick allergy testing device as a virus delivery tool. We show that the Pick can be used to infect the epithelium of the soft palate and the base of the tongue of mice directly, without prior scarification. The ability to induce and track oropharyngeal papillomavirus-induced tumors in the mouse, easily and robustly, will facilitate the study of oropharyngeal tumorigenesis and potential treatments. View Full-Text
Keywords: mouse; papillomavirus; throat; head-and-neck; OPSCC; oropharynx; endoscopy; tumor; MmuPV1; anesthesia mouse; papillomavirus; throat; head-and-neck; OPSCC; oropharynx; endoscopy; tumor; MmuPV1; anesthesia
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Bilger, A.; King, R.E.; Schroeder, J.P.; Piette, J.T.; Hinshaw, L.A.; Kurth, A.D.; AlRamahi, R.W.; Barthel, M.V.; Ward-Shaw, E.T.; Buehler, D.; Masters, K.S.; Thibeault, S.L.; Lambert, P.F. A Mouse Model of Oropharyngeal Papillomavirus-Induced Neoplasia Using Novel Tools for Infection and Nasal Anesthesia. Viruses 2020, 12, 450.

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