Next Article in Journal
Host Determinants of MERS-CoV Transmission and Pathogenesis
Previous Article in Journal
Characterization of the Filovirus-Resistant Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessCommunication
Viruses 2019, 11(3), 279; https://doi.org/10.3390/v11030279

A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire

1
Epidemiology of Highly Pathogenic Microorganisms, Robert Koch Institute, 13353 Berlin, Germany
2
Viral Evolution, Robert Koch Institute, 13353 Berlin, Germany
3
Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10027, USA
4
Institute of Microbiology and Epizootics, Freie Universität Berlin, 14163 Berlin, Germany
5
World Wide Fund for Nature, Dzanga-Sangha Protected Areas, Bangui BP 1053, Central African Republic
6
Laboratoire National D’appui au Développement Agricole/Laboratoire Central de Pathologie Animale, Bingerville BP206, Côte d’Ivoire
*
Authors to whom correspondence should be addressed.
These authors contributed equally to the manuscript.
Received: 28 February 2019 / Revised: 11 March 2019 / Accepted: 16 March 2019 / Published: 19 March 2019
(This article belongs to the Section Animal Viruses)
  |  
PDF [934 KB, uploaded 19 March 2019]
  |  

Abstract

New technologies enable viral discovery in a diversity of hosts, providing insights into viral evolution. We used one such approach, the virome capture sequencing for vertebrate viruses (VirCapSeq-VERT) platform, on 21 samples originating from six dead Maxwell’s duikers (Philantomba maxwellii) from Taï National Park, Côte d’Ivoire. We detected the presence of an orthohepadnavirus in one animal and characterized its 3128 bp genome. The highest viral copy numbers were detected in the spleen, followed by the lung, blood, and liver, with the lowest copy numbers in the kidney and heart; the virus was not detected in the jejunum. Viral copy numbers in the blood were in the range known from humans with active chronic infections leading to liver histolytic damage, suggesting this virus could be pathogenic in duikers, though many orthohepadnaviruses appear to be apathogenic in other hosts, precluding a formal test of this hypothesis. The virus was not detected in 29 other dead duiker samples from the Côte d’Ivoire and Central African Republic, suggesting either a spillover event or a low prevalence in these populations. Phylogenetic analysis placed the virus as a divergent member of the mammalian clade of orthohepadnaviruses, though its relationship to other orthohepadnaviruses remains uncertain. This represents the first orthohepadnavirus described in an artiodactyl. We have tentatively named this new member of the genus Orthohepadnavirus (family Hepadnaviridae), Taï Forest hepadnavirus. Further studies are needed to determine whether it, or some close relatives, are present in a broader range of artiodactyls, including livestock. View Full-Text
Keywords: virus; hepadnavirus; Orthohepadnavirus; discovery; hybridization capture; bovid; artiodactyls; sequencing virus; hepadnavirus; Orthohepadnavirus; discovery; hybridization capture; bovid; artiodactyls; sequencing
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Gogarten, J.F.; Ulrich, M.; Bhuva, N.; Garcia, J.; Jain, K.; Lee, B.; Löhrich, T.; Oleynik, A.; Couacy-Hymann, E.; Fuh Neba, T.; Mishra, N.; Briese, T.; Calvignac-Spencer, S.; Lipkin, W.I.; Leendertz, F.H. A Novel Orthohepadnavirus Identified in a Dead Maxwell’s Duiker (Philantomba maxwellii) in Taï National Park, Côte d’Ivoire. Viruses 2019, 11, 279.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top