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Viruses 2019, 11(2), 161; https://doi.org/10.3390/v11020161

Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice

1
Integrated Research Facility, Division of Clinical Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 8200 Research Plaza, Frederick, MD 27102, USA
2
Biostatistics Research Branch, National Institute of Allergy and Infectious Diseases, 5601 Fishers Lane, Rockville, MD 20852, USA
3
Emerging Viral Pathogens Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 8200 Research Plaza, Frederick, MD 21702, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 14 January 2019 / Revised: 5 February 2019 / Accepted: 13 February 2019 / Published: 16 February 2019
(This article belongs to the Collection Advances in Ebolavirus, Marburgvirus, and Cuevavirus Research)
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Abstract

Following the largest Ebola virus disease outbreak from 2013 to 2016, viral RNA has been detected in survivors from semen and breast milk long after disease recovery. However, as there have been few cases of sexual transmission, it is unclear whether every RNA positive fluid sample contains infectious virus. Virus isolation, typically using cell culture or animal models, can serve as a tool to determine the infectivity of patient samples. However, the sensitivity of these methods has not been assessed for the Ebola virus isolate, Makona. Described here is an efficiency comparison of Ebola virus Makona isolation using Vero E6, Huh-7, monocyte-derived macrophage cells, and suckling laboratory mice. Isolation sensitivity was similar in all methods tested. Laboratory mice and Huh-7 cells were less affected by toxicity from breast milk than Vero E6 and MDM cells. However, the advantages associated with isolation in Huh-7 cells over laboratory mice, including cost effectiveness, sample volume preservation, and a reduction in animal use, make Huh-7 cells the preferred substrate tested for Ebola virus Makona isolation. View Full-Text
Keywords: Mice; Vero; Huh7; Monocyte Derived Macrophages; Virus isolation; Semen; Breast milk; Ebola virus Mice; Vero; Huh7; Monocyte Derived Macrophages; Virus isolation; Semen; Breast milk; Ebola virus
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Logue, J.; Vargas Licona, W.; Cooper, T.K.; Reeder, B.; Byrum, R.; Qin, J.; Deiuliis Murphy, N.; Cong, Y.; Bonilla, A.; Sword, J.; Weaver, W.; Kocher, G.; Olinger, G.G.; Jahrling, P.B.; Hensley, L.E.; Bennett, R.S. Ebola Virus Isolation Using Huh-7 Cells has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB/c Laboratory Mice. Viruses 2019, 11, 161.

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