Next Article in Journal
Safety Studies of Pneumococcal Endolysins Cpl-1 and Pal
Next Article in Special Issue
Proteogenomics Uncovers Critical Elements of Host Response in Bovine Soft Palate Epithelial Cells Following In Vitro Infection with Foot-And-Mouth Disease Virus
Previous Article in Journal
Glycans Controlling Virus Infections: Meeting Report on the 1st International Symposium on Glycovirology Schöntal, Germany, 02–04 May 2018
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle

Base-By-Base Version 3: New Comparative Tools for Large Virus Genomes

1
Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada
2
Center for Global Infectious Disease Research, Seattle Children’s Research Institute, Seattle, WA 98101, USA
3
Department of Pediatrics, University of Washington, Seattle, WA 98195, USA
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(11), 637; https://doi.org/10.3390/v10110637
Received: 25 October 2018 / Revised: 10 November 2018 / Accepted: 13 November 2018 / Published: 15 November 2018
(This article belongs to the Special Issue Virus Bioinformatics)
  |  
PDF [1324 KB, uploaded 15 November 2018]
  |  

Abstract

Base-By-Base is a comprehensive tool for the creation and editing of multiple sequence alignments that is coded in Java and runs on multiple platforms. It can be used with gene and protein sequences as well as with large viral genomes, which themselves can contain gene annotations. This report describes new features added to Base-By-Base over the last 7 years. The two most significant additions are: (1) The recoding and inclusion of “consensus-degenerate hybrid oligonucleotide primers” (CODEHOP), a popular tool for the design of degenerate primers from a multiple sequence alignment of proteins; and (2) the ability to perform fuzzy searches within the columns of sequence data in multiple sequence alignments to determine the distribution of sequence variants among the sequences. The intuitive interface focuses on the presentation of results in easily understood visualizations and providing the ability to annotate the sequences in a multiple alignment with analytic and user data. View Full-Text
Keywords: bioinformatics; virus; comparative genomics; software; Base-By-Base; BBB; poxvirus; ASFV; MSA bioinformatics; virus; comparative genomics; software; Base-By-Base; BBB; poxvirus; ASFV; MSA
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Tu, S.-L.; Staheli, J.P.; McClay, C.; McLeod, K.; Rose, T.M.; Upton, C. Base-By-Base Version 3: New Comparative Tools for Large Virus Genomes. Viruses 2018, 10, 637.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top