Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle
AbstractCreeping plants have unique ornamental value because they have more branches and flowers and the creeping trait is rare in crape myrtle (Lagerstroemia indica L.). In this study, the first filial generation (F1) population was derived from Lagerstroemia fauriei Koehne (standard) and L. indica “Creole” (creeping) and the backcross1 (BC1) population was derived from the backcross of F1 individual S82 (creeping) and L. fauriei. The segregation of the creeping trait was analyzed for 174 seedlings of the BC1 population to examine the linkage relationship between simple sequence repeat (SSR) molecular markers and the creeping trait. Creeping genes were screened using bulked segregant analysis combined with 322 SSR primers, which were detected with good polymorphism. The results show that two SSR markers (S364 and LYS12) were detected, with genetic distances of 23.49 centimorgan (cM) and 25.86 cM from the loci controlling the plant opening angle trait and the branching angle trait, respectively. The accuracy rate for phenotypic verification using S364 and LYS12 was 76.51% and 74.14%, respectively. Our results provide basic information for the molecular marker-assisted selective breeding and cloning of the creeping gene to improve architecture diversity in the breeding of crape myrtle. View Full-Text
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Zheng, T.; Qin, B.; Li, S.; Cai, M.; Pan, H.; Wang, J.; Cheng, T.; Zhang, Q. Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle. Forests 2019, 10, 429.
Zheng T, Qin B, Li S, Cai M, Pan H, Wang J, Cheng T, Zhang Q. Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle. Forests. 2019; 10(5):429.Chicago/Turabian Style
Zheng, Tangchun; Qin, Bo; Li, Suzhen; Cai, Ming; Pan, Huitang; Wang, Jia; Cheng, Tangren; Zhang, Qixiang. 2019. "Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle." Forests 10, no. 5: 429.
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