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Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle

1,2,3,4,5, 1,2,3,4,5,6, 1,2,3,4,5,6, 1,2,3,4,5, 1,2,3,4,5, 1,2,3,4,5, 1,2,3,4,5 and 1,2,3,4,5,6,*
1
Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, Beijing Forestry University, Beijing 100083, China
2
National Engineering Research Center for Floriculture, Beijing Forestry University, Beijing 100083, China
3
Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing 100083, China
4
Engineering Research Center of Landscape Environment of Ministry of Education, Beijing Forestry University, Beijing 100083, China
5
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, Beijing Forestry University, Beijing 100083, China
6
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Forests 2019, 10(5), 429; https://doi.org/10.3390/f10050429
Received: 26 April 2019 / Revised: 11 May 2019 / Accepted: 17 May 2019 / Published: 18 May 2019
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Abstract

Creeping plants have unique ornamental value because they have more branches and flowers and the creeping trait is rare in crape myrtle (Lagerstroemia indica L.). In this study, the first filial generation (F1) population was derived from Lagerstroemia fauriei Koehne (standard) and L. indica “Creole” (creeping) and the backcross1 (BC1) population was derived from the backcross of F1 individual S82 (creeping) and L. fauriei. The segregation of the creeping trait was analyzed for 174 seedlings of the BC1 population to examine the linkage relationship between simple sequence repeat (SSR) molecular markers and the creeping trait. Creeping genes were screened using bulked segregant analysis combined with 322 SSR primers, which were detected with good polymorphism. The results show that two SSR markers (S364 and LYS12) were detected, with genetic distances of 23.49 centimorgan (cM) and 25.86 cM from the loci controlling the plant opening angle trait and the branching angle trait, respectively. The accuracy rate for phenotypic verification using S364 and LYS12 was 76.51% and 74.14%, respectively. Our results provide basic information for the molecular marker-assisted selective breeding and cloning of the creeping gene to improve architecture diversity in the breeding of crape myrtle. View Full-Text
Keywords: Lagerstroemia species; simple sequence repeat markers; bulked segregant analysis; creeping trait; plant architecture Lagerstroemia species; simple sequence repeat markers; bulked segregant analysis; creeping trait; plant architecture
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Zheng, T.; Qin, B.; Li, S.; Cai, M.; Pan, H.; Wang, J.; Cheng, T.; Zhang, Q. Screening of Applicable SSR Molecular Markers Linked to Creeping Trait in Crape Myrtle. Forests 2019, 10, 429.

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