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Open AccessArticle

Horizontal Bilayer for Electrical and Optical Recordings

1
Biophysics, Department of Biology/Chemistry, University Osnabrueck, Barbarastr. 13, Osnabrueck 49076, Germany
2
Ionovation GmbH, Westerbreite 7, Osnabrueck 49084, Germany
*
Author to whom correspondence should be addressed.
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Faßberg 11, Goettingen 37077, Germany
Materials 2012, 5(12), 2705-2730; https://doi.org/10.3390/ma5122705
Received: 15 October 2012 / Revised: 19 November 2012 / Accepted: 4 December 2012 / Published: 10 December 2012
(This article belongs to the Special Issue Supported Lipid Membranes)
Artificial bilayer containing reconstituted ion channels, transporters and pumps serve as a well-defined model system for electrophysiological investigations of membrane protein structure–function relationship. Appropriately constructed microchips containing horizontally oriented bilayers with easy solution access to both sides provide, in addition, the possibility to investigate these model bilayer membranes and the membrane proteins therein with high resolution fluorescence techniques up to the single-molecule level. Here, we describe a bilayer microchip system in which long-term stable horizontal free-standing and hydrogel-supported bilayers can be formed and demonstrate its prospects particularly for single-molecule fluorescence spectroscopy and high resolution fluorescence microscopy in probing the physicochemical properties like phase behavior of the bilayer-forming lipids, as well as in functional studies of membrane proteins. View Full-Text
Keywords: horizontal bilayer; simultaneous electrical-optical recordings; membrane dynamics; lipid diffusion; supported bilayer horizontal bilayer; simultaneous electrical-optical recordings; membrane dynamics; lipid diffusion; supported bilayer
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Bartsch, P.; Walter, C.; Selenschik, P.; Honigmann, A.; Wagner, R. Horizontal Bilayer for Electrical and Optical Recordings. Materials 2012, 5, 2705-2730.

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