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Materials 2017, 10(8), 910;

Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration

4,* and 1,5,*
Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan
Department of Orthopedics, Show-Chwan Memorial Hospital, Changhua 50544, Taiwan
Department of Orthopedic Surgery, Faculty of Medicine, National Yang-Ming University, Taipei 112, Taiwan
Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung 40724, Taiwan
Medical Device Innovation Center, National Cheng Kung University, Tainan 701, Taiwan
Ming-Hsien Hu and Pei-Yuan Lee had equal contribution to this study.
Authors to whom correspondence should be addressed.
Received: 26 June 2017 / Revised: 19 July 2017 / Accepted: 3 August 2017 / Published: 6 August 2017
(This article belongs to the Special Issue Biocompatibility of Materials)
Full-Text   |   PDF [5970 KB, uploaded 6 August 2017]   |  


In this study, we investigated the effect of supplementing a non-dispersive dicalcium phosphate-rich calcium phosphate bone cement (DCP-rich CPC) with type I collagen on in vitro cellular activities and its performance as a bone graft material. Varying amounts of type I collagen were added during the preparation of the DCP-rich CPC. In vitro cell adhesion, morphology, viability, and alkaline phosphatase (ALP) activity were evaluated using progenitor bone cells. Bone graft performance was evaluated via a rat posterolateral lumbar fusion model and osteointegration of the implant. New bone formations in the restorative sites were assessed by micro-computed tomography (micro-CT) and histological analysis. We found that the incorporation of collagen into the DCP-rich CPC was associated with increased cell adhesion, cell viability, and ALP activity in vitro. The spinal fusion model revealed a significant increase in bone regeneration. Additionally, better osseointegration was observed between the host bone and graft with the DCP-rich CPC supplemented with collagen than with the collagen-free DCP-rich CPC control graft. Furthermore, compared to the control graft, the results of micro-CT showed that a smaller amount of residual material was observed with the collagen-containing DCP-rich CPC graft compared with the control graft, which suggests the collagen supplement enhanced new bone formation. Of the different mixtures evaluated in this study (0.8 g DCP-rich CPC supplemented with 0.1, 0.2, and 0.4 mL type I collagen, respectively), DCP-rich CPC supplemented with 0.4 mL collagen led to the highest level of osteogenesis. Our results suggest that the DCP-rich CPC supplemented with collagen has potential to be used as an effective bone graft material in spinal surgery. View Full-Text
Keywords: posterolateral lumbar fusion; non-dispersive calcium phosphate bone cement; collagen; osteogenesis posterolateral lumbar fusion; non-dispersive calcium phosphate bone cement; collagen; osteogenesis

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Hu, M.-H.; Lee, P.-Y.; Chen, W.-C.; Hu, J.-J. Incorporation of Collagen in Calcium Phosphate Cements for Controlling Osseointegration. Materials 2017, 10, 910.

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