Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans
1
Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632014, India
2
CIIMAR/CIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal
3
Department of Biology, Faculty of Sciences, University of Porto, 4069-007 Porto, Portugal
*
Author to whom correspondence should be addressed.
Mar. Drugs 2018, 16(2), 60; https://doi.org/10.3390/md16020060
Received: 27 December 2017 / Revised: 6 February 2018 / Accepted: 9 February 2018 / Published: 12 February 2018
(This article belongs to the Special Issue Screening of Bioactive Compounds from Marine Sources)
Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics.
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Keywords:
actinobacteria; antifungal; polyketide synthase; non ribosomal peptide synthase; Candida albicans
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MDPI and ACS Style
Pavan Kumar, J.G.S.; Gomathi, A.; Vasconcelos, V.; Gothandam, K.M. Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans. Mar. Drugs 2018, 16, 60.
AMA Style
Pavan Kumar JGS, Gomathi A, Vasconcelos V, Gothandam KM. Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans. Marine Drugs. 2018; 16(2):60.
Chicago/Turabian StylePavan Kumar, J. G.S.; Gomathi, Ajitha; Vasconcelos, Vitor; Gothandam, K. M. 2018. "Bioactivity Assessment of Indian Origin—Mangrove Actinobacteria against Candida albicans" Mar. Drugs 16, no. 2: 60.
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