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Comparison of Cell Disruption Methods for Improving Lipid Extraction from Thraustochytrid Strains

Centre for Chemistry and Biotechnology, Geelong Technology Precinct, Deakin University, Waurn Ponds, Geelong 3217, Australia
Author to whom correspondence should be addressed.
Academic Editor: Gilles Barnathan
Mar. Drugs 2015, 13(8), 5111-5127;
Received: 20 June 2015 / Revised: 30 July 2015 / Accepted: 4 August 2015 / Published: 11 August 2015
(This article belongs to the Special Issue Marine Lipids)
PDF [542 KB, uploaded 11 August 2015]


Lipid extraction is an integral part of biodiesel production, as it facilitates the release of fatty acids from algal cells. To utilise thraustochytrids as a potential source for lipid production. We evaluated the extraction efficiency of various solvents and solvent combinations for lipid extraction from Schizochytrium sp. S31 and Thraustochytrium sp. AMCQS5-5. The maximum lipid extraction yield was 22% using a chloroform:methanol ratio of 2:1. We compared various cell disruption methods to improve lipid extraction yields, including grinding with liquid nitrogen, bead vortexing, osmotic shock, water bath, sonication and shake mill. The highest lipid extraction yields were obtained using osmotic shock and 48.7% from Schizochytrium sp. S31 and 29.1% from Thraustochytrium sp. AMCQS5-5. Saturated and monounsaturated fatty acid contents were more than 60% in Schizochytrium sp. S31 which suggests their suitability for biodiesel production. View Full-Text
Keywords: solvents; biodiesel; algae; DHA; marine; omega-3 solvents; biodiesel; algae; DHA; marine; omega-3

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Byreddy, A.R.; Gupta, A.; Barrow, C.J.; Puri, M. Comparison of Cell Disruption Methods for Improving Lipid Extraction from Thraustochytrid Strains. Mar. Drugs 2015, 13, 5111-5127.

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