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Influence of TGFBR2, TGFB3, DNMT1, and DNMT3A Knockdowns on CTGF, TGFBR2, and DNMT3A in Neonatal and Adult Human Dermal Fibroblasts Cell Lines

1
Chair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, 60-781 Poznan, Poland
2
Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Present address: Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland.
Academic Editor: Hany A. El-Shemy
Curr. Issues Mol. Biol. 2021, 43(1), 276-285; https://doi.org/10.3390/cimb43010023
Received: 28 April 2021 / Revised: 29 May 2021 / Accepted: 29 May 2021 / Published: 3 June 2021
(This article belongs to the Section Bioinformatics and Systems Biology Section)
Dermal fibroblasts are responsible for the production of the extracellular matrix that undergoes significant changes during the skin aging process. These changes are partially controlled by the TGF-β signaling, which regulates tissue homeostasis dependently on several genes, including CTGF and DNA methyltransferases. To investigate the potential differences in the regulation of the TGF-β signaling and related molecular pathways at distinct developmental stages, we silenced the expression of TGFB1, TGFB3, TGFBR2, CTGF, DNMT1, and DNMT3A in the neonatal (HDF-N) and adult (HDF-A) human dermal fibroblasts using the RNAi method. Through Western blot, we analyzed the effects of the knockdowns of these genes on the level of the CTGF, TGFBR2, and DNMT3A proteins in both cell lines. In the in vitro assays, we observed that CTGF level was decreased after knockdown of DNMT1 in HDF-N but not in HDF-A. Similarly, the level of DNMT3A was decreased only in HDF-N after silencing of TGFBR2, TGFB3, or DNMT1. TGFBR2 level was lower in HDF-N after knockdown of TGFB3, DNMT1, or DNMT3A, but it was higher in HDF-A after TGFB1 silencing. The reduction of TGFBR2 after silencing of DNMT3A and vice versa in neonatal cells only suggests the developmental stage-specific interactions between these two genes. However, additional studies are needed to explain the dependencies between analyzed proteins. View Full-Text
Keywords: TGFB1; TGFB2; TGFB3; TGFBR2; CTGF; DNMT3A; DNMT1; human dermal fibroblasts TGFB1; TGFB2; TGFB3; TGFBR2; CTGF; DNMT3A; DNMT1; human dermal fibroblasts
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MDPI and ACS Style

Tomela, K.; Karolak, J.A.; Ginter-Matuszewska, B.; Kabza, M.; Gajecka, M. Influence of TGFBR2, TGFB3, DNMT1, and DNMT3A Knockdowns on CTGF, TGFBR2, and DNMT3A in Neonatal and Adult Human Dermal Fibroblasts Cell Lines. Curr. Issues Mol. Biol. 2021, 43, 276-285. https://doi.org/10.3390/cimb43010023

AMA Style

Tomela K, Karolak JA, Ginter-Matuszewska B, Kabza M, Gajecka M. Influence of TGFBR2, TGFB3, DNMT1, and DNMT3A Knockdowns on CTGF, TGFBR2, and DNMT3A in Neonatal and Adult Human Dermal Fibroblasts Cell Lines. Current Issues in Molecular Biology. 2021; 43(1):276-285. https://doi.org/10.3390/cimb43010023

Chicago/Turabian Style

Tomela, Katarzyna, Justyna A. Karolak, Barbara Ginter-Matuszewska, Michal Kabza, and Marzena Gajecka. 2021. "Influence of TGFBR2, TGFB3, DNMT1, and DNMT3A Knockdowns on CTGF, TGFBR2, and DNMT3A in Neonatal and Adult Human Dermal Fibroblasts Cell Lines" Current Issues in Molecular Biology 43, no. 1: 276-285. https://doi.org/10.3390/cimb43010023

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