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Volume 26
Issue 1
10.21775/cimb.026.065
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Current Issues in Molecular Biology is published by MDPI from Volume 43 Issue 1 (2021). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Caister Press.
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Review

Improving CRISPR-Cas9 On-Target Specificity

by
Muhammad Jamal
1,*,
Arif Ullah
2,
Muhammad Ahsan
1,
Rohit Tyagi
1,
Zeshan Habib
1 and
Khaista Rehman
1
1
State Key Laboratory of Agricultural Microbiology, College of Animal Science and Medicine, Huazhong Agricultural University, Wuhan, China
2
Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences Lahore, Pakistan
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2018, 26(1), 65-80; https://doi.org/10.21775/cimb.026.065
Submission received: 1 June 2017 / Revised: 6 July 2017 / Accepted: 5 August 2017 / Published: 7 September 2017

Abstract

The CRISPR/Cas9 has revolutionized the field of molecular biology, medical genetics and medicine. The technology is robust, facile and simple to achieve genome targeting in cells and organisms. However, to propagate these nucleases for therapeutic application, the on-target specificity is of paramount importance. Although the binding and cleavage of off-target sites by Cas9 is an issue of concern, however, the specificity of CRISPR technology is greatly improved in current research employing the use of engineer nucleases, improved gRNA selection, novel Cas9 orthologues and the advancement in methods to detect and screen offtarget sites and its effects. Here we summarize the advances in this state-of-the-art technology that will equip the genome editing tools to be applied in clinical research. The researcher should optimize these methods with emphasis to achieving perfection in the specificity.

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MDPI and ACS Style

Jamal, M.; Ullah, A.; Ahsan, M.; Tyagi, R.; Habib, Z.; Rehman, K. Improving CRISPR-Cas9 On-Target Specificity. Curr. Issues Mol. Biol. 2018, 26, 65-80. https://doi.org/10.21775/cimb.026.065

AMA Style

Jamal M, Ullah A, Ahsan M, Tyagi R, Habib Z, Rehman K. Improving CRISPR-Cas9 On-Target Specificity. Current Issues in Molecular Biology. 2018; 26(1):65-80. https://doi.org/10.21775/cimb.026.065

Chicago/Turabian Style

Jamal, Muhammad, Arif Ullah, Muhammad Ahsan, Rohit Tyagi, Zeshan Habib, and Khaista Rehman. 2018. "Improving CRISPR-Cas9 On-Target Specificity" Current Issues in Molecular Biology 26, no. 1: 65-80. https://doi.org/10.21775/cimb.026.065

APA Style

Jamal, M., Ullah, A., Ahsan, M., Tyagi, R., Habib, Z., & Rehman, K. (2018). Improving CRISPR-Cas9 On-Target Specificity. Current Issues in Molecular Biology, 26(1), 65-80. https://doi.org/10.21775/cimb.026.065

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