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Review

Expressing Active Ribozymes in Cells

by
Dmitry Samarsky
1,
Gerardo Ferbeyre
2 and
Edouard Bertrand
3,*
1
HHMI/University of Massachusetts Medical Center, Program in Molecular Medicine, 373 Plantation Street, Worcester, MA 01605, USA
2
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA
3
IGMM-CNRS, 1919, Route de Mende, BP 5051, Montpellier, France
*
Author to whom correspondence should be addressed.
Curr. Issues Mol. Biol. 2000, 2(3), 87-93; https://doi.org/10.21775/cimb.002.087
Submission received: 10 March 2000 / Revised: 8 May 2000 / Accepted: 8 June 2000 / Published: 1 July 2000

Abstract

Artificially engineered ribozymes can be used to specifically regulate expression of target genes. Such ribozymes can be synthesized chemically and delivered into the cell exogeneously. Alternatively, ribozymes can be produced by the cell endogenously, after introduction of the artificial gene into the cellular genome. In the latter case, the design of the artificial gene defines the ribozyme properties, such as: expression level, intracellular localization, folding and association with proteins. Generally speaking, design of the expression vector is critical to obtain active ribozyme molecules. This paper first describes factors that are known or predicted to affect ribozyme activity in the cell, then reviews various expression systems that have been specifically developed for ribozymes. Lastly, a recently developed ribozyme system termed snorbozymes (small nucleolar RNA:ribozyme hybrids) will be discussed. This powerful test system has generated several important observations that are likely to affect the future development of ribozyme technology.

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MDPI and ACS Style

Samarsky, D.; Ferbeyre, G.; Bertrand, E. Expressing Active Ribozymes in Cells. Curr. Issues Mol. Biol. 2000, 2, 87-93. https://doi.org/10.21775/cimb.002.087

AMA Style

Samarsky D, Ferbeyre G, Bertrand E. Expressing Active Ribozymes in Cells. Current Issues in Molecular Biology. 2000; 2(3):87-93. https://doi.org/10.21775/cimb.002.087

Chicago/Turabian Style

Samarsky, Dmitry, Gerardo Ferbeyre, and Edouard Bertrand. 2000. "Expressing Active Ribozymes in Cells" Current Issues in Molecular Biology 2, no. 3: 87-93. https://doi.org/10.21775/cimb.002.087

APA Style

Samarsky, D., Ferbeyre, G., & Bertrand, E. (2000). Expressing Active Ribozymes in Cells. Current Issues in Molecular Biology, 2(3), 87-93. https://doi.org/10.21775/cimb.002.087

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