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Open AccessArticle

Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements

1
Forschungs- und Technologiezentrum Westküste der Universität Kiel, Hafentörn 1, 25761 Büsum, Germany
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BlueBioTech GmbH, Hafentörn 1, 25761 Büsum, Germany
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Zentrum für Biochemie und Molekularbiologie der Universität Kiel, Leibnizstraße 11, 24098 Kiel, Germany
*
Author to whom correspondence should be addressed.
Sensors 2007, 7(10), 2080-2095; https://doi.org/10.3390/s7102080
Received: 20 June 2007 / Accepted: 1 October 2007 / Published: 3 October 2007
(This article belongs to the Special Issue Physiological Sensing)
The demonstrated modified spectrophotometric method makes use of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and its specific absorbance properties. Theabsorbance decreases when the radical is reduced by antioxidants. In contrast to otherinvestigations, the absorbance was measured at a wavelength of 550 nm. This wavelengthenabled the measurements of the stable free DPPH radical without interference frommicroalgal pigments. This approach was applied to methanolic microalgae extracts for twodifferent DPPH concentrations. The changes in absorbance measured vs. the concentrationof the methanolic extract resulted in curves with a linear decrease ending in a saturationregion. Linear regression analysis of the linear part of DPPH reduction versus extractconcentration enabled the determination of the microalgae’s methanolic extractsantioxidative potentials which was independent to the employed DPPH concentrations. Theresulting slopes showed significant differences (6 - 34 μmol DPPH g-1 extractconcentration) between the single different species of microalgae (Anabaena sp.,Isochrysis galbana, Phaeodactylum tricornutum, Porphyridium purpureum, Synechocystissp. PCC6803) in their ability to reduce the DPPH radical. The independency of the signal on the DPPH concentration is a valuable advantage over the determination of the EC50 value. View Full-Text
Keywords: absorbance decrease; antioxidative potential; microalgae; radical scavenging; linear regression analysis; spectrophotometric approach absorbance decrease; antioxidative potential; microalgae; radical scavenging; linear regression analysis; spectrophotometric approach
MDPI and ACS Style

Marxen, K.; Vanselow, K.H.; Lippemeier, S.; Hintze, R.; Ruser, A.; Hansen, U.-P. Determination of DPPH Radical Oxidation Caused by Methanolic Extracts of Some Microalgal Species by Linear Regression Analysis of Spectrophotometric Measurements. Sensors 2007, 7, 2080-2095.

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