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Review

Fluorescence Polarization-Based Bioassays: New Horizons

1
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia
2
Department of Chemical Enzymology, Chemical Faculty, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia
*
Author to whom correspondence should be addressed.
Sensors 2020, 20(24), 7132; https://doi.org/10.3390/s20247132
Received: 14 November 2020 / Revised: 7 December 2020 / Accepted: 9 December 2020 / Published: 12 December 2020
(This article belongs to the Special Issue Fluorescent Sensors)
Fluorescence polarization holds considerable promise for bioanalytical systems because it allows the detection of selective interactions in real time and a choice of fluorophores, the detection of which the biosample matrix does not influence; thus, their choice simplifies and accelerates the preparation of samples. For decades, these possibilities were successfully applied in fluorescence polarization immunoassays based on differences in the polarization of fluorophore emissions excited by plane-polarized light, whether in a free state or as part of an immune complex. However, the results of recent studies demonstrate the efficacy of fluorescence polarization as a detected signal in many bioanalytical methods. This review summarizes and comparatively characterizes these developments. It considers the integration of fluorescence polarization with the use of alternative receptor molecules and various fluorophores; different schemes for the formation of detectable complexes and the amplification of the signals generated by them. New techniques for the detection of metal ions, nucleic acids, and enzymatic reactions based on fluorescence polarization are also considered. View Full-Text
Keywords: fluorescence polarization; immunoassay; rotation of molecules; bioreceptors; antibodies; aptamers; nucleic acids; switched on biosensors; portable optical detectors fluorescence polarization; immunoassay; rotation of molecules; bioreceptors; antibodies; aptamers; nucleic acids; switched on biosensors; portable optical detectors
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MDPI and ACS Style

Hendrickson, O.D.; Taranova, N.A.; Zherdev, A.V.; Dzantiev, B.B.; Eremin, S.A. Fluorescence Polarization-Based Bioassays: New Horizons. Sensors 2020, 20, 7132. https://doi.org/10.3390/s20247132

AMA Style

Hendrickson OD, Taranova NA, Zherdev AV, Dzantiev BB, Eremin SA. Fluorescence Polarization-Based Bioassays: New Horizons. Sensors. 2020; 20(24):7132. https://doi.org/10.3390/s20247132

Chicago/Turabian Style

Hendrickson, Olga D., Nadezhda A. Taranova, Anatoly V. Zherdev, Boris B. Dzantiev, and Sergei A. Eremin. 2020. "Fluorescence Polarization-Based Bioassays: New Horizons" Sensors 20, no. 24: 7132. https://doi.org/10.3390/s20247132

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