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Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase

1
School of Engineering and Material Science, Queen Mary University of London, Mile End Road, London E1 4NS, UK
2
Centre of Foundation Studies, Universiti Teknologi MARA, Cawangan Selangor, Kampus Dengkil, Dengkil, Selangor 43800, Malaysia
3
Institute of Medical Engineering, School of Basic Medical Sciences, Xi’an Jiaotong University Health Science Center, Xi’an 710061, China
4
The Lennard-Jones Laboratories, School of Chemical and Physical Sciences, Keele University, Staffordshire ST5 5BG, UK
5
Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK
*
Author to whom correspondence should be addressed.
This paper is an extended version of the conference paper: Collagenase Biosensor Based on the Degradation of Peptide Cross-Linked Poly(Ethylene Glycol) Hydrogel Films. In Proceedings of the EUROSENSORS 2018, Graz, Austria, 9–12 September 2018.
Sensors 2019, 19(7), 1677; https://doi.org/10.3390/s19071677
Received: 31 January 2019 / Revised: 28 March 2019 / Accepted: 2 April 2019 / Published: 8 April 2019
(This article belongs to the Special Issue Eurosensors 2018 Selected Papers)
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Abstract

Peptide cross-linked poly(ethylene glycol) hydrogel has been widely used for drug delivery and tissue engineering. However, the use of this material as a biosensor for the detection of collagenase has not been explored. Proteases play a key role in the pathology of diseases such as rheumatoid arthritis and osteoarthritis. The detection of this class of enzyme using the degradable hydrogel film format is promising as a point-of-care device for disease monitoring. In this study, a protease biosensor was developed based on the degradation of a peptide cross-linked poly(ethylene glycol) hydrogel film and demonstrated for the detection of collagenase. The hydrogel was deposited on gold-coated quartz crystals, and their degradation in the presence of collagenase was monitored using a quartz crystal microbalance (QCM). The biosensor was shown to respond to concentrations between 2 and 2000 nM in less than 10 min with a lower detection limit of 2 nM. View Full-Text
Keywords: protease biosensor; collagenase; poly(ethylene glycol) norbornene; QCM; hydrogel degradation; peptide cross-links; click chemistry protease biosensor; collagenase; poly(ethylene glycol) norbornene; QCM; hydrogel degradation; peptide cross-links; click chemistry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Ahmad, N.; Colak, B.; Zhang, D.-W.; Gibbs, M.J.; Watkinson, M.; Becer, C.R.; Gautrot, J.E.; Krause, S. Peptide Cross-Linked Poly (Ethylene Glycol) Hydrogel Films as Biosensor Coatings for the Detection of Collagenase. Sensors 2019, 19, 1677.

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