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3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection

1
Institute of Complex Systems Bioelectronics (ICS-8), Forschungszentrum Jülich, 52425 Jülich, Germany
2
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, 52074 Aachen, Germany
*
Authors to whom correspondence should be addressed.
Sensors 2019, 19(1), 148; https://doi.org/10.3390/s19010148
Received: 30 November 2018 / Revised: 21 December 2018 / Accepted: 28 December 2018 / Published: 3 January 2019
(This article belongs to the Special Issue Immunosensors - 2018 Trends and Perspective)
For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated. View Full-Text
Keywords: frequency mixing magnetic detection; 3D print; magnetic sandwich immunoasssay; additive manufacturing; magnetic bead frequency mixing magnetic detection; 3D print; magnetic sandwich immunoasssay; additive manufacturing; magnetic bead
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Achtsnicht, S.; Tödter, J.; Niehues, J.; Telöken, M.; Offenhäusser, A.; Krause, H.-J.; Schröper, F. 3D Printed Modular Immunofiltration Columns for Frequency Mixing-Based Multiplex Magnetic Immunodetection. Sensors 2019, 19, 148.

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