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Open AccessArticle

Electrochemical DNA Sensor Based on Carbon Black—Poly(Neutral Red) Composite for Detection of Oxidative DNA Damage

1
A.M. Butlerov’ Chemistry Institute of Kazan Federal University, 18 Kremlevskaya Street, Kazan 420008, Russia
2
Department of Nuclear Physics and Biophysics, Comenius University, Mlynska dolina F1, 842 48 Bratislava, Slovakia
*
Authors to whom correspondence should be addressed.
Sensors 2018, 18(10), 3489; https://doi.org/10.3390/s18103489
Received: 26 September 2018 / Revised: 11 October 2018 / Accepted: 14 October 2018 / Published: 16 October 2018
(This article belongs to the Special Issue Membrane-Based Biosensing)
Voltammetric DNA sensor has been proposed on the platform of glassy carbon electrode covered with carbon black with adsorbed pillar[5]arene molecules. Electropolymerization of Neutral Red performed in the presence of native or oxidatively damaged DNA resulted in formation of hybrid material which activity depended on the DNA conditions. The assembling of the surface layer was confirmed by scanning electron microscopy and electrochemical impedance spectroscopy. The influence of DNA and pillar[5]arene on redox activity of polymeric dye was investigated and a significant increase of the peak currents was found for DNA damaged by reactive oxygen species generated by Cu2+/H2O2 mixture. Pillar[5]arene improves the electron exchange conditions and increases the response and its reproducibility. The applicability of the DNA sensor developed was shown on the example of ascorbic acid as antioxidant. It decreases the current in the concentration range from 1.0 μM to 1.0 mM. The possibility to detect antioxidant activity was qualitatively confirmed by testing tera infusion. The DNA sensor developed can find application in testing of carcinogenic species and searching for new antitumor drugs. View Full-Text
Keywords: DNA sensor; oxidative DNA damage; electropolymerization; poly(Neutral Red); electrochemical impedance spectroscopy DNA sensor; oxidative DNA damage; electropolymerization; poly(Neutral Red); electrochemical impedance spectroscopy
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MDPI and ACS Style

Kuzin, Y.; Kappo, D.; Porfireva, A.; Shurpik, D.; Stoikov, I.; Evtugyn, G.; Hianik, T. Electrochemical DNA Sensor Based on Carbon Black—Poly(Neutral Red) Composite for Detection of Oxidative DNA Damage. Sensors 2018, 18, 3489.

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