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Open AccessArticle

A Nanoporous Alumina Membrane Based Electrochemical Biosensor for Histamine Determination with Biofunctionalized Magnetic Nanoparticles Concentration and Signal Amplification

1
Institute of Ocean Research, Zhejiang University of Technology, Hangzhou 310014, China
2
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China
3
Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, HungHom, Kowloon, Hong Kong, China
*
Author to whom correspondence should be addressed.
Academic Editors: Anton Ficai and Alexandru Mihai Grumezescu
Sensors 2016, 16(10), 1767; https://doi.org/10.3390/s16101767
Received: 15 September 2016 / Revised: 10 October 2016 / Accepted: 17 October 2016 / Published: 22 October 2016
(This article belongs to the Special Issue Nanobiosensors in Food Industry)
Histamine is an indicator of food quality and indispensable in the efficient functioning of various physiological systems. Rapid and sensitive determination of histamine is urgently needed in food analysis and clinical diagnostics. Traditional histamine detection methods require qualified personnel, need complex operation processes, and are time-consuming. In this study, a biofunctionalized nanoporous alumina membrane based electrochemical biosensor with magnetic nanoparticles (MNPs) concentration and signal amplification was developed for histamine determination. Nanoporous alumina membranes were modified by anti-histamine antibody and integrated into polydimethylsiloxane (PDMS) chambers. The specific antibody modified MNPs were used to concentrate histamine from samples and transferred to the antibody modified nanoporous membrane. The MNPs conjugated to histamine were captured in the nanopores via specific reaction between histamine and anti-histamine antibody, resulting in a blocking effect that was amplified by MNPs in the nanopores. The blockage signals could be measured by electrochemical impedance spectroscopy across the nanoporous alumina membrane. The sensing platform had great sensitivity and the limit of detection (LOD) reached as low as 3 nM. This biosensor could be successfully applied for histamine determination in saury that was stored in frozen conditions for different hours, presenting a potentially novel, sensitive, and specific sensing system for food quality assessment and safety support. View Full-Text
Keywords: nanoporous alumina membrane; histamine; magnetic nanoparticles; electrochemical biosensor nanoporous alumina membrane; histamine; magnetic nanoparticles; electrochemical biosensor
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MDPI and ACS Style

Ye, W.; Xu, Y.; Zheng, L.; Zhang, Y.; Yang, M.; Sun, P. A Nanoporous Alumina Membrane Based Electrochemical Biosensor for Histamine Determination with Biofunctionalized Magnetic Nanoparticles Concentration and Signal Amplification. Sensors 2016, 16, 1767.

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