Next Article in Journal
Anomaly Detection Based on Sensor Data in Petroleum Industry Applications
Next Article in Special Issue
Development of a Passive Liquid Valve (PLV) Utilizing a Pressure Equilibrium Phenomenon on the Centrifugal Microfluidic Platform
Previous Article in Journal
Surveying Multidisciplinary Aspects in Real-Time Distributed Coding for Wireless Sensor Networks
Previous Article in Special Issue
Micro-Fabricated DC Comparison Calorimeter for RF Power Measurement
Open AccessArticle

Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System

1
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
2
Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China
3
Department of Mechanical Engineering, University of Alberta, Edmonton, AB T6G 2G8, Canada
*
Authors to whom correspondence should be addressed.
Sensors 2015, 15(2), 2763-2773; https://doi.org/10.3390/s150202763
Received: 6 December 2014 / Revised: 12 January 2015 / Accepted: 19 January 2015 / Published: 27 January 2015
(This article belongs to the Special Issue On-Chip Sensors)
This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young’s modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties. View Full-Text
Keywords: microfluidics; single-cell analysis; cellular biophysics; instantaneous Young’s modulus; specific membrane capacitance microfluidics; single-cell analysis; cellular biophysics; instantaneous Young’s modulus; specific membrane capacitance
MDPI and ACS Style

Zhao, Y.; Chen, D.; Luo, Y.; Chen, F.; Zhao, X.; Jiang, M.; Yue, W.; Long, R.; Wang, J.; Chen, J. Simultaneous Characterization of Instantaneous Young’s Modulus and Specific Membrane Capacitance of Single Cells Using a Microfluidic System. Sensors 2015, 15, 2763-2773.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop